Nishimoto Mana, Umezawa Misuzu, Okuyama Hisashi, Kumano Norikuni, Nomura Tetsuro, Takahashi Jun-Ichi
Faculty of Life Sciences, Kyoto Sangyo University, Kyoto, Japan.
Department of Agro-environmental Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
Mitochondrial DNA B Resour. 2018 Mar 14;3(1):354-356. doi: 10.1080/23802359.2018.1450673.
The complete is mitochondrial genome of the Japanese bumblebee from Hokkaido Island, Japan is analysed using next generation sequencing. The mitochondrial genome of was observed to be a circular molecule of 15,835 bp. The average AT content in the mitochondrial genome was 85.53%. It was predicted to contain 13 protein-coding genes (PCGs), 22 tRNA genes, and two rRNA genes, along with one A + T-rich control region. The PCGs had ATA, ATG, or ATT as the initiation codon and were terminated by the typical stop codon TAA, except for . All the tRNA genes typically formed a cloverleaf secondary structure, except for , , and . The molecular phylogenetic analysis indicated that the from Hokkaido Island population was most similar to that of the geographically isolated from Rebun Island.
利用下一代测序技术分析了来自日本北海道岛的日本大黄蜂的完整线粒体基因组。观察到该线粒体基因组是一个15,835 bp的环状分子。该线粒体基因组的平均AT含量为85.53%。预计它包含13个蛋白质编码基因(PCGs)、22个tRNA基因和两个rRNA基因,以及一个富含A+T的控制区。PCGs以ATA、ATG或ATT作为起始密码子,并由典型的终止密码子TAA终止,但[此处缺失信息]除外。除了[此处缺失信息]、[此处缺失信息]和[此处缺失信息]外,所有tRNA基因通常形成三叶草二级结构。分子系统发育分析表明,北海道岛种群的[此处缺失信息]与来自礼文岛地理隔离的[此处缺失信息]最为相似。