基于线粒体基因序列推断尼泊尔东方家鼠（ ）的新记录
New record of the Oriental house rat, , in Nepal inferred from mitochondrial gene sequences.
作者信息
Adhikari Pradeep, Han Sang-Hyun, Kim Yoo-Kyung, Kim Tae-Wook, Thapa Tej Bahadur, Subedi Naresh, Kunwar Amar, Banjade Maniram, Oh Hong-Shik
机构信息
Faculty of Science Education, Jeju National University, Jeju, Republic of Korea.
National Institute of Ecology, Seocheon, Republic of Korea.
出版信息
Mitochondrial DNA B Resour. 2018 Mar 21;3(1):386-390. doi: 10.1080/23802359.2018.1436991.
This study determines the presence of . from in Nepal using morphological and molecular analyses. Morphologically, it is indistinguishable with owing to similar fur colour and morphometric data. However, molecular identification and phylogenetic analysis using sequences of the mitochondrial DNA (mtDNA) () gene revealed two different species . and . from collected specimens. The genetic distance between . and . was found 0.043. In phylogenetic tree, the clade of . is distinguished into two sub-clades, . found in Nepal, and East Asian countries, China, Laos, Thailand, Viet Nam, and South Korea have genetic distance 0.031, suggesting the different lineages of . . This study confirmed the . present in Nepal. Our findings suggest that morphological analysis and molecular study should be carried out simultaneously for accurate identification of small sized cryptic mammals like . and . .
本研究通过形态学和分子分析确定尼泊尔境内[具体物种名称未给出]的存在情况。在形态学上，由于毛色和形态测量数据相似，它与[另一相似物种名称未给出]难以区分。然而，使用线粒体DNA（mtDNA）[具体基因名称未给出]基因序列进行的分子鉴定和系统发育分析显示，从采集的标本中发现了两个不同的物种，即[物种一名称]和[物种二名称]。[物种一名称]和[物种二名称]之间的遗传距离为0.043。在系统发育树中，[物种一名称]的分支分为两个亚分支，在尼泊尔发现的[亚分支一名称]与东亚国家中国、老挝、泰国、越南和韩国的[亚分支一名称]遗传距离为0.031，这表明[物种一名称]存在不同的谱系。本研究证实了尼泊尔存在[物种名称]。我们的研究结果表明，对于像[物种一名称]和[物种二名称]这样的小型隐秘哺乳动物，应同时进行形态学分析和分子研究以进行准确鉴定。
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