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通过二代测序揭示的风铃草科某植物的完整叶绿体基因组序列及其系统发育意义

The complete chloroplast genome sequence of (Campanulaceae) revealed by next-generation sequencing and phylogenetic implication.

作者信息

Wang Yu Chao, Zhou Ya Fu, Chen Chen, Mao Shao Li

机构信息

Xi'an Botanical Garden of Shaanxi Province/Institute of Botany of Shaanxi Province, Xi'an, China.

出版信息

Mitochondrial DNA B Resour. 2018 Apr 10;3(1):450-451. doi: 10.1080/23802359.2018.1462117.

Abstract

The complete chloroplast genome sequence of was determined by Illumina pair-end sequencing. The results showed that the complete plastid genome was 169,419 bp in length, containing a large single copy (LSC) of 85,599 bp and a small single copy (SSC) of 8054 bp, which were separated by a pair of 37,883 bp inverted repeats (IRs). A total of 109 unique genes were annotated, including 75 protein coding genes, 30 tRNA genes, and 4 rRNA genes. Among these genes, 16 genes contained one or two introns. The overall GC contents of the plastid genome were 38.4%. A maximum likelihood phylogenetic analysis showed that and formed one clade at the base of the phylogenetic tree of Campanulaceae with a high support value.

摘要

通过Illumina双末端测序确定了[物种名称]的完整叶绿体基因组序列。结果表明,完整的质体基因组长度为169,419 bp,包含一个85,599 bp的大单拷贝(LSC)和一个8054 bp的小单拷贝(SSC),它们被一对37,883 bp的反向重复序列(IRs)隔开。总共注释了109个独特基因,包括75个蛋白质编码基因、30个tRNA基因和4个rRNA基因。在这些基因中,16个基因含有一个或两个内含子。质体基因组的总体GC含量为38.4%。最大似然系统发育分析表明,[物种名称]和[另一物种名称]在桔梗科系统发育树的基部形成一个分支,支持值很高。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/967d/7800722/b4301e2ba6ac/TMDN_A_1462117_F0001_B.jpg

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