Ru Binghua, Wang Ting, Liu Yongfeng, Zhao Xiaochao, Lei Ming
Shanghai Origingene Biotechnology Company Limited, Shang Hai, China.
GeneMind Biosciences Company Limited, Shen Zhen, China.
Mitochondrial DNA B Resour. 2024 May 2;9(5):578-582. doi: 10.1080/23802359.2024.2347511. eCollection 2024.
Leibnitzia anandria is a perennial herbaceous plant with medicinal properties, and the entire plant can be used in traditional medicine. Leibnitzia anandria was once classified under the genus Gerbera Cass., but was reclassified under Leibnitzia Cass. recently. In this study, using the GeneLab M sequencing technology of the Genemind platform, we have sequenced, assembled, and analyzed the complete chloroplast genome of Leibnitzia anandria for the first time. The genome is 154168 bp in length, consisting of a large single-copy region(LSC, 80166 bp), a small single-copy region(SSC, 18202 bp), and a pair of inverted repeat sequences(IR, 27900 bp). We have predicted and annotated a total of 133 genes, including 88 protein-coding genes, 37 tRNA-coding genes, and 8 rRNA-coding genes. The results of the phylogenetic analysis indicate that Leibnitzia anandria and Leibnitzia nepalensis, as well as the closely related Gerbera plant, clustered into a separate clade, rather than grouping together with the other plants belonging to the tribe Mutisieae. This study provides new information for the phylogeny research of Leibnitzia anandria, contributing to a better understanding of its taxonomy and evolution.
无叶菜是一种具有药用特性的多年生草本植物,其全株可用于传统医学。无叶菜曾被归类于大丁草属(Gerbera Cass.),但最近被重新归类到无叶菜属(Leibnitzia Cass.)。在本研究中,我们首次使用Genemind平台的GeneLab M测序技术对无叶菜的完整叶绿体基因组进行了测序、组装和分析。该基因组长度为154168 bp,由一个大单拷贝区域(LSC,80166 bp)、一个小单拷贝区域(SSC,18202 bp)和一对反向重复序列(IR,27900 bp)组成。我们总共预测并注释了133个基因,包括88个蛋白质编码基因、37个tRNA编码基因和8个rRNA编码基因。系统发育分析结果表明,无叶菜和尼泊尔无叶菜以及与之密切相关的大丁草属植物聚为一个单独的分支,而不是与菊科帚菊木族的其他植物归为一类。本研究为无叶菜的系统发育研究提供了新信息,有助于更好地理解其分类和进化。