Wang Shouhong, Liu Lusha, Zhang Meihua, Jiang Jianping
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.
Department of Herpetology, Chengdu Institute of Biology, Chinese Academy of Sciences, Beijing, China.
Mitochondrial DNA B Resour. 2018 Apr 28;3(2):547-548. doi: 10.1080/23802359.2018.1467238.
The first complete mitochondrial genome of a frog was characterized in this work. The mitogenome was 17,061 base pairs (bp) in length, containing 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes, and a control region (D-loop). The overall base composition was 29.65% A, 30.77% T, 25.41% C and 14.17% G. Besides, the gene arrangement was identical to that observed in vertebrates. Five of 13 PCGs (, , , and ) were ended with incomplete stop codon T. Except for gene encoded on L-strand, all other PCGs were encoded on H-strand. The non-coding region was 1665 bp in size, which was heavily biased to A + T (65.77%). Additionally, we found mitogenome size of all sequenced species were bigger than that of species, which was ascribe to the difference of D-loop size. Phylogenetic analysis showed that was the sister species of . This work will provide basic molecular data for further molecular evolution and phylogenetic research of and other microhylids.
在这项研究中首次对一种青蛙的完整线粒体基因组进行了特征分析。该线粒体基因组长度为17,061个碱基对（bp），包含13个蛋白质编码基因（PCGs）、2个rRNA基因、22个tRNA基因和一个控制区（D环）。总体碱基组成为29.65%A、30.77%T、25.41%C和14.17%G。此外，基因排列与在脊椎动物中观察到 的相同。13个PCGs中的5个（ 、 、 、 和 ）以不完整的终止密码子T结尾。除了在L链上编码的 基因外，所有其他PCGs都在H链上编码。非编码区大小为1665bp，严重偏向于A+T（65.77%）。此外，我们发现所有已测序的 物种的线粒体基因组大小都比 物种的大，这归因于D环大小 的差异。系统发育分析表明， 是 的姐妹物种。这项工作将为 及其他姬蛙科动物的进一步分子进化和系统发育研究提供基础分子数据。
