Deng Xuyan, Wang Shouhong, Liang Xixi, Jiang Jianping, Wang Bin, Deng Liangji
a College of Resources and Environment, Sichuan Agricultural University , Chengdu , China .
b Key Laboratory of Land Information in Sichuan Province , Chengdu , China .
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3391-2. doi: 10.3109/19401736.2015.1018235. Epub 2015 May 11.
We determined the complete mitochondrial genome of Kaloula rugifera in this work. The mitogenome was 17,073 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region (D-loop). The base composition of the light strand was 29.7% A, 30.4% T, 25.7% C and 14.2% G. The gene order and contents of it is identical to most amphibian mitogenome. All protein-coding genes began with ATG as start codon except ND1 gene beginning with GTG and COI gene beginning with ATA. Five protein-coding genes (COII, ATP 6, COIII, ND3 and ND4) ended with incomplete stop codon T. The 22 tRNA genes with the size ranging from 65 bp to 73 bp were interspersed along the whole genome. The D-loop region containing tandem repetition was 1675 bp in length and heavily biased to A + T nucleotides.
在本研究中,我们测定了皱皮姬蛙的完整线粒体基因组。该线粒体基因组长度为17,073 bp,包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和一个控制区(D-loop)。轻链的碱基组成为29.7%A、30.4%T、25.7%C和14.2%G。其基因顺序和内容与大多数两栖动物线粒体基因组相同。除了以GTG开头的ND1基因和以ATA开头的COI基因外,所有蛋白质编码基因均以ATG作为起始密码子。五个蛋白质编码基因(COII、ATP 6、COIII、ND3和ND4)以不完整的终止密码子T结束。22个tRNA基因大小在65 bp至73 bp之间,散布于整个基因组中。含有串联重复序列的D-loop区长度为1675 bp,且A+T核苷酸严重偏向。