Mota Thaís Fernandes Mendonça, Fabrin Thomaz Mansini Carrenho, Gasques Luciano Seraphim, Ortêncio Filho Henrique, Prioli Alberto José, Prioli Sônia Maria Alves Pinto
Programa de Pós-Graduação em Biologia Comparada, Universidade Estadual de Maringá, Maringá, Brazil.
Programa de Pós-Graduação em Ecologia de Ambientes Aquáticos Continentais, Universidade Estadual de Maringá, Maringá, Brazil.
Mitochondrial DNA B Resour. 2018 Jul 10;3(2):758-762. doi: 10.1080/23802359.2018.1484261.
Bat populations are declining worldwide. Accurate identification is essential to promote species' conservation. However, minimal morphological differences and a high rate of cryptic species make identification difficult, unless voucher specimens are kept, a controversial issue today. The objective of this work was to standardize a method of extracting non-lethal DNA using bats' uropatagium micro-tissue, aiming the molecular identification of species that occur in the region of Maringá PR. The method standardized was efficient, and does not cause serious damage to bats. For future field studies, collection of micro-tissue and morphometry of the specimens will be sufficient for accurate identification.
全球蝙蝠种群数量正在下降。准确识别对于促进物种保护至关重要。然而，极小的形态差异和高比例的隐性物种使得识别变得困难，除非保存凭证标本，而这在当今是一个有争议的问题。这项工作的目的是规范一种使用蝙蝠尿膜微组织提取非致死性DNA的方法，旨在对巴拉那州马林加地区出现的物种进行分子鉴定。所规范的方法是有效的，并且不会对蝙蝠造成严重损害。对于未来的野外研究，收集微组织和对标本进行形态测量将足以进行准确识别。
