Arakawa Kazuharu
Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan.
Faculty of Environment and Information Studies, Keio University, Fujisawa, Japan.
Mitochondrial DNA B Resour. 2018 Aug 1;3(2):810-811. doi: 10.1080/23802359.2018.1495118.
The complete mitchondrial genome of , a cosmopolitan heterotardigrade collected in Japan, has been sequenced using a single molecule real-time (SMRT) sequencing long read after whole genome amplification from a single individual. The genome has a total length of 15,817 bp, consisting of 13 protein-coding genes, 20 tRNA, 2 rRNA genes, and an AT-rich control region. The nucleotide composition was extremely AT-rich, with 78.41% AT. Being in a different class of the phylum Tardigrada, there is a large sequence divergence from the previously reported mitochondrial genome of the eutardigrade , but the gene order of protein-coding genes are mostly conserved. This is the first report of a complete mitochondrial genome of a heterotardigrade.
从日本采集的一种世界性异缓步动物的完整线粒体基因组,在对单个个体进行全基因组扩增后,使用单分子实时(SMRT)测序长读段进行了测序。该基因组全长15,817 bp,由13个蛋白质编码基因、20个tRNA、2个rRNA基因和一个富含AT的控制区组成。核苷酸组成极度富含AT,AT含量为78.41%。作为缓步动物门的不同类别,它与先前报道的真缓步动物的线粒体基因组存在很大的序列差异,但蛋白质编码基因的基因顺序大多保守。这是异缓步动物完整线粒体基因组的首次报道。