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利用下一代测序技术对岩石扇贝()完整线粒体基因组进行表征及系统发育分析。

Characterization and phylogenetic analysis of the complete mitochondrial genome from Rock Scallop () using next-generation sequencing.

作者信息

Liao Dejie, Zhou Ying, Tong Jingou, Cao Shanmao, Yu Xiaomu, Fu Beide, Yang Dazuo

机构信息

Liaoning Shellfish Fine Breed Breeding Engineering Technology Research Center, Dalian Ocean University, DaLian, China.

University of Chinese Academy of Sciences, Beijing, China.

出版信息

Mitochondrial DNA B Resour. 2018 Jul 27;3(2):827-828. doi: 10.1080/23802359.2018.1483752.

Abstract

In this study, the complete 18,495 bp mitochondrial genome was determined from Rock scallop () using next-generation sequencing technology. The complete mitochondrial genome contained 12 protein-coding genes (PCGs), 2 ribosomal RNA genes, 23 transfer RNA genes, without and , which was similar with most mitochondrial genomes of marine bivalve molluscs. Gene annotations, including gene order, genetic code, start and stop codons and codons bias, were identified. Phylogenetic tree was constructed using Neighbor-Joining (NJ) method based on the PCGs showed the present species clustered within the Pteriomorphia clade. This work should be of importance not only for the better understanding of the relationships within Pectinidae, but also for the development of useful genetic markers in Rock scallop aquaculture and restoration efforts.

摘要

在本研究中,利用下一代测序技术从岩石扇贝()中测定了完整的18495bp线粒体基因组。完整的线粒体基因组包含12个蛋白质编码基因(PCGs)、2个核糖体RNA基因、23个转移RNA基因,没有和,这与大多数海洋双壳贝类的线粒体基因组相似。确定了基因注释,包括基因顺序、遗传密码、起始和终止密码子以及密码子偏好。基于PCGs使用邻接法(NJ)构建的系统发育树表明,当前物种聚集在翼形亚纲分支内。这项工作不仅对于更好地理解扇贝科内部的关系具有重要意义,而且对于岩石扇贝养殖和恢复工作中有用遗传标记的开发也具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9fe/7799609/1ac3c6a07aba/TMDN_A_1483752_F0001_B.jpg

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