Wang Ying, Wen Hanyu, Yao Jiyuan, Sun Kaixun, Wang Wenbo, Liu Hongyan, Yang Dong, Zhang Fanrong, Xiong Fei
College of Life Sciences, Jianghan University, Wuhan, PR China.
Mitochondrial DNA B Resour. 2018 Oct 30;3(2):1040-1041. doi: 10.1080/23802359.2018.1511840.
The complete mitochondrial DNA genome of the Salangid icefish () was sequenced by the primer walking sequence method. The entire mitochondrial genome of this species is 17,035 bp in length, making it the longest among the reported mitochondrial genomes of . It contains 13 protein-coding genes, 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and one control region (CR). The gene arrangement, nucleotide composition, and codon usage pattern of the mitochondrial genome are similar to those of other teleosts except for two long tandem repeats in the CR. A 486 bp tandem repeat fragment was identified that comprises 2 copies of 243 bp motif and accounts approximately 35.5% of the CR. The 243 bp tandem repeat motif can be folded into a stem-loop secondary structure. Phylogenetic analysis based on 12 concatenated protein-coding genes of the heavy strand shows the genus diverged most recently and clustered with as a clade.
通过引物步移测序法对冰鱼的完整线粒体DNA基因组进行了测序。该物种的整个线粒体基因组长度为17,035 bp,是已报道的冰鱼线粒体基因组中最长的。它包含13个蛋白质编码基因、2个核糖体RNA(rRNA)基因、22个转运RNA(tRNA)基因和一个控制区(CR)。线粒体基因组的基因排列、核苷酸组成和密码子使用模式与其他硬骨鱼相似,但CR中有两个长串联重复序列。鉴定出一个486 bp的串联重复片段,它由2个243 bp基序的拷贝组成,约占CR的35.5%。243 bp的串联重复基序可折叠成茎环二级结构。基于重链12个串联蛋白质编码基因的系统发育分析表明,该属最近分化,与作为一个分支聚集在一起。