Zhou Min, Wang Cheng, Xu Ziyue, Peng Zhicun, He Yang, Wang Ying
Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, Jianghan University, Wuhan, China Jianghan University Wuhan China.
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China Institute of Hydrobiology, Chinese Academy of Sciences Wuhan China.
Zookeys. 2024 Dec 10;1221:51-69. doi: 10.3897/zookeys.1221.129136. eCollection 2024.
In this study, the complete mitochondrial genome of was first determined by the primer walking sequence method. The complete mitochondrial genome was 16,574 bp in length, including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, and a control region (D-loop). The gene arrangement pattern was identical to that of other teleosts. The overall base composition was 29.9% A, 28.5% T, 25.5% C, and 16.1% G, with an A+T bias of 58.4%. Furthermore, phylogenetic analyses were conducted based on 13 PCGs from the mitochondrial genomes of 18 cobitid species using with three different methods (Neighbor-joining, Maximum likelihood, and Bayesian inference). All methods consistently showed that the four species of the genus form a monophyletic group. This study would provide effective molecular information for the species as well as novel genetic marker for the study of species identification.
在本研究中，首次通过引物步移测序法确定了[物种名称]的完整线粒体基因组。完整的线粒体基因组长度为16,574 bp，包括13个蛋白质编码基因（PCGs）、22个转移RNA（tRNA）基因、两个核糖体RNA（rRNA）基因和一个控制区（D-loop）。基因排列模式与其他硬骨鱼相同。总体碱基组成为29.9%的A、28.5%的T、25.5%的C和16.1%的G，A+T偏倚为58.4%。此外，基于18种鳅科物种线粒体基因组中的13个PCGs，采用三种不同方法（邻接法、最大似然法和贝叶斯推断法）进行了系统发育分析。所有方法均一致表明，[属名]的四个物种形成一个单系群。本研究将为[物种名称]提供有效的分子信息，并为物种鉴定研究提供新的遗传标记。
