Wang Lin, Sui Changhai, Wang Ying, Guo Jia
Jilin Engineering Vocational College, Siping City, Jilin Province, P.R. China.
School of Food Production Technology and Biotechnology, Changchun Vocational Institute of Technology, Changchun City, Jilin Province, P.R. China.
Mitochondrial DNA B Resour. 2018 Oct 26;3(2):1256-1258. doi: 10.1080/23802359.2018.1532352.
Chloroplast (cp) genome sequences provide a valuable source for phylogenetic analysis, which becomes a popular tool for population and phylogeny in a recent report. Here, the complete chloroplast genome of the has been reconstructed from the whole-genome Illumina sequencing data. The circular genome is 169,820 bp in size and comprises a pair of inverted repeat (IR) regions of 33,641 bp each, a large single-copy (LSC) region of 98,872 bp and a small single-copy (SSC) region of 3666 bp. The total GC content is 33.8%, while the corresponding values of the IR regions, LSC, and SSC and are 38.7%, 31.7% and 26.7%, respectively. The chloroplast genome contains 151 genes, including 91 protein-coding genes, eight ribosomal RNA genes and 52 transfer RNA genes. Two genes ( and ) are pseudogenized or lost in its cpDNA. The maximum-likelihood phylogenetic analysis showed a close relationship with in Orchidaceae. Our findings provide useful information for phylogenetic and evolutionary research of species.
叶绿体（cp）基因组序列为系统发育分析提供了宝贵的资源，在最近的一份报告中，它成为了用于种群和系统发育研究的流行工具。在这里，已从全基因组Illumina测序数据中重建了该物种的完整叶绿体基因组。这个环状基因组大小为169,820 bp，由两个各为33,641 bp的反向重复（IR）区域、一个98,872 bp的大单拷贝（LSC）区域和一个3666 bp的小单拷贝（SSC）区域组成。总GC含量为33.8%，而IR区域、LSC和SSC的相应值分别为38.7%、31.7%和26.7%。叶绿体基因组包含151个基因，包括91个蛋白质编码基因、8个核糖体RNA基因和52个转运RNA基因。有两个基因（和）在其叶绿体DNA中已假基因化或丢失。最大似然系统发育分析表明它与兰科中的某一物种关系密切。我们的研究结果为该物种的系统发育和进化研究提供了有用的信息。
需注意，原文中“the complete chloroplast genome of the has been reconstructed”这里“the has been”中间缺失了具体物种信息，翻译时只能按照原文呈现。
