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(兰科)的完整叶绿体基因组。

The complete chloroplast genome of (Orchidaceae).

作者信息

Sui Changhai, Liu Shuying, Liu Huimin, Liu Hongzhang

机构信息

College of Life sciences, Jilin Agricultural University, Changchun City, P. R. China.

Jilin Engineering Vocational college, Siping City, Jilin, P.R. China.

出版信息

Mitochondrial DNA B Resour. 2018 Jul 27;3(2):820-822. doi: 10.1080/23802359.2018.1483779.

DOI:10.1080/23802359.2018.1483779
PMID:33474335
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7799620/
Abstract

Chloroplast (cp) genome sequences have become a useful tool for phylogeneitc and evolutionary study in recent reports. Here the complete chloroplast genome of the has been reconstructed from the whole-genome Illumina sequencing data. The circular genome is 162,127 bp in size, and comprises of a pair of inverted repeat (IR) regions of 31,457 bp each, a large single-copy (LSC) region of 91,711 bp, and a small single-copy (SSC) region of 7,502 bp. The total GC content is 35.4%, while the corresponding values of the LSC, SSC, and IR regions are 32.7%, 26.9%, and 40.9%, respectively. The chloroplast genome contains 133 genes, including 87 protein-coding genes, eight ribosomal RNA genes, and 38 transfer RNA genes. Three genes (, and ) are pseudogenized or lost in its cpDNA. The Maximum-Likelihood phylogenetic analysis showed a close relationship with in Orchidaceae. Our findings provide useful information for phylogenetic and evolutionary research of species.

摘要

叶绿体(cp)基因组序列在最近的报道中已成为系统发育和进化研究的有用工具。在此,已从全基因组Illumina测序数据中重建了[物种名称]的完整叶绿体基因组。环状基因组大小为162,127 bp,由两个各为31,457 bp的反向重复(IR)区域、一个91,711 bp的大单拷贝(LSC)区域和一个7,502 bp的小单拷贝(SSC)区域组成。总GC含量为35.4%,而LSC、SSC和IR区域的相应值分别为32.7%、26.9%和40.9%。叶绿体基因组包含133个基因,包括87个蛋白质编码基因、8个核糖体RNA基因和38个转移RNA基因。三个基因([基因名称1]、[基因名称2]和[基因名称3])在其cpDNA中被假基因化或丢失。最大似然系统发育分析表明与兰科的[物种名称]关系密切。我们的研究结果为[物种名称]物种的系统发育和进化研究提供了有用信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389a/7799620/7427ecbc9f20/TMDN_A_1483779_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389a/7799620/7427ecbc9f20/TMDN_A_1483779_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/389a/7799620/7427ecbc9f20/TMDN_A_1483779_F0001_B.jpg

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引用本文的文献

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本文引用的文献

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The complete chloroplast genome of the Dendrobium strongylanthum (Orchidaceae: Epidendroideae).硬叶石斛(兰科:树兰亚科)的完整叶绿体基因组
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):3048-9. doi: 10.3109/19401736.2015.1063128. Epub 2015 Jul 8.
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MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.MEGA6:分子进化遗传学分析版本 6.0。
Mol Biol Evol. 2013 Dec;30(12):2725-9. doi: 10.1093/molbev/mst197. Epub 2013 Oct 16.
3
Reconstructing mitochondrial genomes directly from genomic next-generation sequencing reads--a baiting and iterative mapping approach.
直接从基因组下一代测序读段重建线粒体基因组——一种诱饵和迭代映射方法。
Nucleic Acids Res. 2013 Jul;41(13):e129. doi: 10.1093/nar/gkt371. Epub 2013 May 9.
4
The complete chloroplast genome sequence of Citrus sinensis (L.) Osbeck var 'Ridge Pineapple': organization and phylogenetic relationships to other angiosperms.脐橙(Citrus sinensis (L.) Osbeck var 'Ridge Pineapple')的完整叶绿体基因组序列:结构及其与其他被子植物的系统发育关系
BMC Plant Biol. 2006 Sep 30;6:21. doi: 10.1186/1471-2229-6-21.