Diacci Chiara, Abedi Tayebeh, Lee Jee Woong, Gabrielsson Erik O, Berggren Magnus, Simon Daniel T, Niittylä Totte, Stavrinidou Eleni
Laboratory of Organic Electronics, Department of Science and Technology, Linköping University, 601 74 Norrköping, Sweden.
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Via Campi 103, 41125 Modena, Italy.
iScience. 2020 Dec 17;24(1):101966. doi: 10.1016/j.isci.2020.101966. eCollection 2021 Jan 22.
Bioelectronic devices that convert biochemical signals to electronic readout enable biosensing with high spatiotemporal resolution. These technologies have been primarily applied in biomedicine while in plants sensing is mainly based on invasive methods that require tissue sampling, hindering in-vivo detection and having poor spatiotemporal resolution. Here, we developed enzymatic biosensors based on organic electrochemical transistors (OECTs) for in-vivo and real-time monitoring of sugar fluctuations in the vascular tissue of trees. The glucose and sucrose OECT-biosensors were implanted into the vascular tissue of trees and were operated through a low-cost portable unit for 48hr. Our work consists a proof-of-concept study where implantable OECT-biosensors not only allow real-time monitoring of metabolites in plants but also reveal new insights into diurnal sugar homeostasis. We anticipate that this work will contribute to establishing bioelectronic technologies as powerful minimally invasive tools in plant science, agriculture and forestry.
将生化信号转换为电子读数的生物电子设备能够实现具有高时空分辨率的生物传感。这些技术主要应用于生物医学领域，而在植物中，传感主要基于需要组织采样的侵入性方法，这阻碍了体内检测且时空分辨率较差。在此，我们开发了基于有机电化学晶体管（OECT）的酶生物传感器，用于对树木维管组织中的糖分波动进行体内实时监测。葡萄糖和蔗糖OECT生物传感器被植入树木的维管组织中，并通过一个低成本便携式装置运行48小时。我们的工作构成了一项概念验证研究，其中可植入的OECT生物传感器不仅能够实时监测植物中的代谢物，还能揭示昼夜糖稳态的新见解。我们预计这项工作将有助于将生物电子技术确立为植物科学、农业和林业中强大的微创工具。
