School of Life Science and Technology, Henan Collaborative Innovation Center in Modern Biological Breeding, Henan Institute of Science and Technology, Xinxiang, China.
Shandong Drug and Food Vocational College, Weihai, China.
J Sci Food Agric. 2021 Aug 30;101(11):4613-4620. doi: 10.1002/jsfa.11104. Epub 2021 Feb 17.
The non-grain crop cassava has attracted intense attention in the biorefinery process. However, efficient biorefinery of whole cassava is faced with some challenges due to the existence of strain inhibition and refractory cellulose during the citrate production process.
Here, a novel breeding method - atmospheric and room temperature plasma (ARTP) - was applied for strain improvement of citrate-producing strain Aspergillus niger from whole cassava. The citrate yield of the mutant obtained using ARTP mutagenesis increased by 36.5% in comparison with the original strain. Moreover, citric acid fermentation was further improved on the basis of an enhanced co-saccharification strategy by supplementing glucoamylase and cellulase. The fermentation efficiency increased by 35.8% with a 17.0 g L reduction in residual sugar on a pilot scale.
All these results confirmed that a combination of the novel breeding method and enhanced co-saccharification strategy could be used to efficiently refine whole cassava. The results also provide inspiration for the production of value-added products and waste disposal in agro-based industries. © 2021 Society of Chemical Industry.
在生物炼制过程中，非粮作物木薯受到了极大的关注。然而，由于在柠檬酸生产过程中存在菌株抑制和纤维素难处理的问题，高效的全木薯生物炼制仍面临一些挑战。
本研究采用常压室温等离子体（ARTP）对从全木薯中生产柠檬酸的黑曲霉进行了新型的育种方法。与原始菌株相比，ARTP 诱变获得的突变株的柠檬酸产量提高了 36.5%。此外，通过补充糖化酶和纤维素酶，进一步改进了基于协同糖化增强的柠檬酸发酵策略。在中试规模下，发酵效率提高了 35.8%，残糖量减少了 17.0 g/L。
所有这些结果都证实，新型育种方法和协同糖化增强策略的结合可以有效地精制全木薯。这些结果也为农业产业中增值产品的生产和废物处理提供了启示。 © 2021 英国化学学会。
