Li Yansheng, Men Xiujin, Gao Guowei, Tian Ye, Wen Yongqiang, Zhang Xueji
Beijing Key Laboratory for Sensors, Beijing Information Science & Technology University, Beijing 100192, P.R. China.
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, P.R. China.
Lab Chip. 2021 Feb 23;21(4):719-724. doi: 10.1039/d0lc01147a.
Distance-based detection methods with a quantitative readout are of great significance to point-of-care testing (POCT), are low-cost and user-friendly, and can be integrated into portable analytical devices. Here, we submit a visual quantitative distance-based sensor by capillary force alteration in a capillary tube. This sensor converts the wettability alteration caused by the target molecules into a capillary rise height signal. Moreover, the sensor profits from isothermal amplification technology, achieving the detection of miRNAs with high sensitivity and specificity by visually reading the height of the water in the capillary tube. The proposed biosensor shows great potential in routine clinical diagnosis as well as POCT in resource-limited settings.
具有定量读数的基于距离的检测方法对于即时检测（POCT）具有重要意义，成本低且用户友好，并且可以集成到便携式分析设备中。在此，我们通过毛细管中毛细管力的改变提出了一种可视化定量基于距离的传感器。该传感器将目标分子引起的润湿性改变转化为毛细管上升高度信号。此外，该传感器受益于等温扩增技术，通过直观读取毛细管中水的高度实现了对miRNA的高灵敏度和特异性检测。所提出的生物传感器在常规临床诊断以及资源有限环境中的即时检测中显示出巨大潜力。
