Medical Ward 20, Lianshui County People's Hospital, Huai'an, China.
Department of Respiratory Medicine, Hongze District People's Hospital, Huai'an, China.
Environ Toxicol. 2021 Jun;36(6):1052-1060. doi: 10.1002/tox.23104. Epub 2021 Jan 21.
Circular RNAs (circRNAs) are associated with lung cancer progression. However, it is unclear whether and how circRNA hsa_circ_0001073 (circ_0001073) are involved in lung cancer progression. circ_0001073, microRNA (miR)-626, and leukemia inhibitory factor receptor (LIFR) abundances were determined via quantitative reverse transcription polymerase chain reaction or western blot. Cell viability, invasion, and apoptosis were analyzed by cell counting kit-8, transwell analysis and flow cytometry, respectively. The target correlation was tested by dual-luciferase reporter analysis or RNA immunoprecipitation. Results showed that circ_0001073 abundance was down-regulated in lung cancer cells. circ_0001073 constrained cell viability and invasion and facilitated apoptosis in lung cancer cells. miR-626 was targeted via circ_0001073, and circ_0001073 inhibited lung cancer progression via reducing miR-626 expression. LIFR was targeted via miR-626, and miR-626 knockdown constrained cell viability and invasion and facilitated apoptosis in lung cancer cells via up-regulating LIFR. circ_0001073 increased LIFR expression via miR-626 in lung cancer cells. In conclusion, circ_0001073 represses lung cancer progression via miR-626/LIFR axis, indicating the potential value of circ_0001073 in lung cancer treatment.
环状 RNA（circRNAs）与肺癌的进展有关。然而，circRNA hsa_circ_0001073（circ_0001073）是否以及如何参与肺癌的进展尚不清楚。通过定量逆转录聚合酶链反应或 Western blot 测定 circ_0001073、microRNA（miR）-626 和白血病抑制因子受体（LIFR）的丰度。通过细胞计数试剂盒-8、Transwell 分析和流式细胞术分别分析细胞活力、侵袭和细胞凋亡。通过双荧光素酶报告分析或 RNA 免疫沉淀测试靶标相关性。结果表明，circ_0001073 在肺癌细胞中的丰度下调。circ_0001073 限制肺癌细胞的活力和侵袭并促进细胞凋亡。circ_0001073 通过靶向 miR-626，通过降低 miR-626 的表达来抑制肺癌的进展。LIFR 通过 miR-626 靶向，miR-626 敲低通过上调 LIFR 限制肺癌细胞的活力和侵袭并促进细胞凋亡。circ_0001073 通过 miR-626 在肺癌细胞中增加 LIFR 的表达。总之，circ_0001073 通过 miR-626/LIFR 轴抑制肺癌的进展，表明 circ_0001073 在肺癌治疗中的潜在价值。
