舒芬太尼预处理通过 miR-125a/DRAM2 轴保护心肌缺血/再灌注损伤。
Sufentanil preconditioning protects against myocardial ischemia/reperfusion injury via miR-125a/DRAM2 axis.
机构信息
Department of Anesthesiology, The First Hospital Affiliated to Jinzhou Medical University , Jinzhou, P.R. China.
出版信息
Cell Cycle. 2021 Feb;20(4):383-391. doi: 10.1080/15384101.2021.1875668. Epub 2021 Jan 21.
Abstract
This project aimed to investigate the protective mechanism of sufentanil pretreatment on myocardial ischemia-reperfusion injury (IRI). An rat model of myocardial IRI and an cultured cardiomyocyte model of hypoxia-reoxygenation (H/R) were used to confirm the anti-oxidation and anti-autophagy effects of sufentanil. The interaction between miR-125a and damage-regulated autophagy regulator 2 (DRAM2) was verified by luciferase reporter assay. We showed that pretreatment with sufentanil suppressed myocardial damage caused by IRI in rats by inhibiting oxidative stress and mitochondrial autophagy. Furthermore, the cardioprotective mechanism of sufentanil was mediated by miR-125a. MiR-125a targeted DRAM2 to ameliorate cardiomyocyte autophagy and oxidative injury following H/R treatment. In conclusion, our results demonstrated that sufentanil pretreatment produced a protective effect against myocardial IRI via regulating miR-125a/DRAM2 signaling axis.
摘要
本项目旨在探究舒芬太尼预处理对心肌缺血再灌注损伤（IRI）的保护机制。采用大鼠心肌 IRI 模型和体外培养的心肌细胞缺氧复氧（H/R）模型，证实舒芬太尼的抗氧化和抗自噬作用。通过荧光素酶报告实验验证 miR-125a 与损伤调节自噬调节剂 2（DRAM2）之间的相互作用。结果表明，舒芬太尼预处理通过抑制氧化应激和线粒体自噬抑制大鼠 IRI 引起的心肌损伤。此外，舒芬太尼的心脏保护机制是通过 miR-125a 介导的。miR-125a 通过靶向 DRAM2 减轻 H/R 处理后心肌细胞的自噬和氧化损伤。综上所述，本研究结果表明，舒芬太尼预处理通过调节 miR-125a/DRAM2 信号通路对心肌 IRI 产生保护作用。
相似文献
1
Sufentanil preconditioning protects against myocardial ischemia/reperfusion injury via miR-125a/DRAM2 axis.舒芬太尼预处理通过 miR-125a/DRAM2 轴保护心肌缺血/再灌注损伤。
Cell Cycle. 2021 Feb;20(4):383-391. doi: 10.1080/15384101.2021.1875668. Epub 2021 Jan 21.
2
Luteolin Inhibits Ischemia/Reperfusion-Induced Myocardial Injury in Rats via Downregulation of microRNA-208b-3p.木犀草素通过下调微小RNA-208b-3p抑制大鼠缺血/再灌注诱导的心肌损伤。
PLoS One. 2015 Dec 14;10(12):e0144877. doi: 10.1371/journal.pone.0144877. eCollection 2015.
3
MicroRNA-370 protects against myocardial ischemia/reperfusion injury in mice following sevoflurane anesthetic preconditioning through PLIN5-dependent PPAR signaling pathway.七氟醚预处理通过 PLIN5 依赖的 PPAR 信号通路对小鼠心肌缺血/再灌注损伤的保护作用与 microRNA-370 有关。
Biomed Pharmacother. 2019 May;113:108697. doi: 10.1016/j.biopha.2019.108697. Epub 2019 Mar 9.
4
MicroRNA-34a protects myocardial cells against ischemia-reperfusion injury through inhibiting autophagy via regulating TNFα expression.微小RNA-34a通过调节肿瘤坏死因子α（TNFα）的表达抑制自噬，从而保护心肌细胞免受缺血-再灌注损伤。
Biochem Cell Biol. 2018 Jun;96(3):349-354. doi: 10.1139/bcb-2016-0158. Epub 2017 May 25.
5
Epigallocatechin gallate prevents mitochondrial impairment and cell apoptosis by regulating miR-30a/p53 axis.没食子酸表没食子儿茶素酯通过调控 miR-30a/p53 轴防止线粒体损伤和细胞凋亡。
Phytomedicine. 2019 Aug;61:152845. doi: 10.1016/j.phymed.2019.152845. Epub 2019 Jan 30.
6
SIRT1 activation by curcumin pretreatment attenuates mitochondrial oxidative damage induced by myocardial ischemia reperfusion injury.姜黄素预处理激活 SIRT1 减轻心肌缺血再灌注损伤引起的线粒体氧化损伤。
Free Radic Biol Med. 2013 Dec;65:667-679. doi: 10.1016/j.freeradbiomed.2013.07.007. Epub 2013 Jul 20.
7
Remifentanil protects against myocardial ischemia/reperfusion injury via miR-205-mediated regulation of PINK1.瑞芬太尼通过 miR-205 介导的调控 PINK1 保护心肌缺血/再灌注损伤。
J Toxicol Sci. 2021;46(6):263-271. doi: 10.2131/jts.46.263.
8
miR-202-5p protects rat against myocardial ischemia reperfusion injury by downregulating the expression of Trpv2 to attenuate the Ca overload in cardiomyocytes.miR-202-5p 通过下调 TRPV2 的表达来减轻心肌细胞内钙超载，从而保护大鼠免受心肌缺血再灌注损伤。
J Cell Biochem. 2019 Aug;120(8):13680-13693. doi: 10.1002/jcb.28641. Epub 2019 May 6.
9
Sevoflurane protects cardiomyocytes against hypoxia/reperfusion injury via LINC01133/miR-30a-5p axis.七氟醚通过 LINC01133/miR-30a-5p 轴保护心肌细胞免受缺氧/再灌注损伤。
Biosci Rep. 2020 Dec 23;40(12). doi: 10.1042/BSR20200713.
10
[Effect of sufentanil preconditioning on myocardial P-Akt expression in rats during myocardial ischemia-reperfusion].舒芬太尼预处理对大鼠心肌缺血再灌注时心肌P-Akt表达的影响
Nan Fang Yi Ke Da Xue Xue Bao. 2014 Mar;34(3):335-40.
引用本文的文献
1
2-aminoethoxydiphenylborane intervenes intracellular calcium signaling and attenuates myocardial ischemia-reperfusion injury in mice through ITPR1/MCU pathway.2-氨基乙氧基二苯硼烷通过ITPR1/MCU途径干预细胞内钙信号传导并减轻小鼠心肌缺血再灌注损伤。
Sci Rep. 2025 Aug 12;15(1):29469. doi: 10.1038/s41598-025-14822-2.
2
The Impact of Opioids on Epigenetic Modulation in Myocardial Ischemia and Reperfusion Injury: Focus on Non-coding RNAs.阿片类药物对心肌缺血再灌注损伤中表观遗传调控的影响：聚焦于非编码RNA
J Cardiovasc Transl Res. 2025 Apr 8. doi: 10.1007/s12265-025-10609-y.
3
Sufentanil Suppresses Cell Carcinogenesis Via Targeting miR-186-5p/HMGB1 Axis and Wnt/β-Catenin Pathway in Non-Small-Cell Lung Cancer.舒芬太尼通过靶向非小细胞肺癌中的miR-186-5p/HMGB1轴和Wnt/β-连环蛋白通路抑制细胞癌变。
Mol Biotechnol. 2025 Mar;67(3):1054-1064. doi: 10.1007/s12033-024-01104-x. Epub 2024 Mar 12.
4
Crosstalk among Reactive Oxygen Species, Autophagy and Metabolism in Myocardial Ischemia and Reperfusion Stages.活性氧、自噬和代谢在心肌缺血和再灌注阶段的相互作用。
Aging Dis. 2024 May 7;15(3):1075-1107. doi: 10.14336/AD.2023.0823-4.
5
A bibliometric analysis of myocardial ischemia/reperfusion injury from 2000 to 2023.2000年至2023年心肌缺血/再灌注损伤的文献计量分析。
Front Cardiovasc Med. 2023 Jun 13;10:1180792. doi: 10.3389/fcvm.2023.1180792. eCollection 2023.
6
Sufentanil inhibits Pin1 to attenuate renal tubular epithelial cell ischemia-reperfusion injury by activating the PI3K/AKT/FOXO1 pathway.舒芬太尼通过激活 PI3K/AKT/FOXO1 通路抑制 Pin1 从而减轻肾小管上皮细胞缺血再灌注损伤。
Int Urol Nephrol. 2023 Aug;55(8):1903-1916. doi: 10.1007/s11255-023-03651-9. Epub 2023 Jun 10.
7
Remifentanil but not sufentanil induces cardioprotection in human ischemic heart muscle in vitro.瑞芬太尼而非舒芬太尼诱导体外人缺血心肌的心脏保护作用。
BMC Pharmacol Toxicol. 2023 Apr 20;24(1):25. doi: 10.1186/s40360-023-00660-3.
8
Sufentanil promotes autophagy and improves ischemiareperfusioninduced acute kidney injury via upregulating microRNA145.舒芬太尼通过上调 microRNA145 促进自噬，改善缺血再灌注诱导的急性肾损伤。
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2022 Oct 28;47(10):1315-1323. doi: 10.11817/j.issn.1672-7347.2022.210602.
9
Targeted Mitochondrial Drugs for Treatment of Ischemia-Reperfusion Injury.用于治疗缺血再灌注损伤的靶向线粒体药物
Curr Drug Targets. 2022;23(16):1526-1536. doi: 10.2174/1389450123666220913121422.
10
Sufentanil Alleviates Sepsis-Induced Myocardial Injury and Stress Response in Rats through the ERK/GSK-3 Signaling Axis.舒芬太尼通过ERK/GSK-3信号轴减轻大鼠脓毒症诱导的心肌损伤和应激反应。
Evid Based Complement Alternat Med. 2022 Jun 21;2022:9630716. doi: 10.1155/2022/9630716. eCollection 2022.
本文引用的文献
1
MicroRNA-146a protects against myocardial ischaemia reperfusion injury by targeting Med1.MicroRNA-146a 通过靶向 Med1 保护心肌缺血再灌注损伤。
Cell Mol Biol Lett. 2019 Nov 27;24:62. doi: 10.1186/s11658-019-0186-5. eCollection 2019.
2
Sufentanil impairs autophagic degradation and inhibits cell migration in NCI-H460 .舒芬太尼损害自噬降解并抑制NCI-H460细胞的迁移。
Oncol Lett. 2019 Dec;18(6):6829-6835. doi: 10.3892/ol.2019.10997. Epub 2019 Oct 17.
3
Oxidative Stress and Autophagy.氧化应激与自噬。
Adv Exp Med Biol. 2019;1206:179-198. doi: 10.1007/978-981-15-0602-4_9.
4
MicroRNA-145 Protects against Myocardial Ischemia Reperfusion Injury via CaMKII-Mediated Antiapoptotic and Anti-Inflammatory Pathways.MicroRNA-145 通过 CaMKII 介导的抗凋亡和抗炎途径保护心肌缺血再灌注损伤。
Oxid Med Cell Longev. 2019 Sep 10;2019:8948657. doi: 10.1155/2019/8948657. eCollection 2019.
5
Levels of miR-125a-5p are altered in Mycobacterium avium-infected macrophages and associate with the triggering of an autophagic response.分枝杆菌感染的巨噬细胞中 miR-125a-5p 的水平发生改变，并与自噬反应的触发有关。
Microbes Infect. 2020 Jan-Feb;22(1):31-39. doi: 10.1016/j.micinf.2019.07.002. Epub 2019 Jul 23.
6
pPolyHb protects myocardial H9C2 cells against ischemia-reperfusion injury by regulating the Pink1-Parkin-mediated mitochondrial autophagy pathway.聚血红素通过调控 Pink1-Parkin 介导的线粒体自噬通路保护心肌 H9C2 细胞对抗缺血再灌注损伤。
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):1248-1255. doi: 10.1080/21691401.2019.1594243.
7
Sufentanil Preconditioning Protects Against Hepatic Ischemia-Reperfusion Injury by Suppressing Inflammation.舒芬太尼预处理通过抑制炎症反应保护肝脏缺血再灌注损伤。
Med Sci Monit. 2019 Mar 28;25:2265-2273. doi: 10.12659/MSM.913145.
8
miRNA-125a modulates autophagy of thyroiditis through PI3K/Akt/mTOR signaling pathway.微小RNA-125a通过PI3K/Akt/mTOR信号通路调节甲状腺炎的自噬。
Exp Ther Med. 2019 Apr;17(4):2465-2472. doi: 10.3892/etm.2019.7256. Epub 2019 Feb 12.
9
miR‑146a regulates the function of Th17 cell differentiation to modulate cervical cancer cell growth and apoptosis through NF‑κB signaling by targeting TRAF6.miR-146a 通过靶向 TRAF6 调控 NF-κB 信号通路调节 Th17 细胞分化功能从而抑制宫颈癌细胞生长和凋亡。
Oncol Rep. 2019 May;41(5):2897-2908. doi: 10.3892/or.2019.7046. Epub 2019 Mar 7.
10
Myocardial Ischemia Reperfusion Injury: Apoptotic, Inflammatory and Oxidative Stress Role of Galectin-3.心肌缺血再灌注损伤：半乳糖凝集素-3的凋亡、炎症及氧化应激作用
Cell Physiol Biochem. 2018;50(3):1123-1139. doi: 10.1159/000494539. Epub 2018 Oct 24.
Zotero 插件