环介导等温扩增 (LAMP)：一种用于快速诊断冠状病毒 SARS-CoV-2 的有效分子即时检测技术。
Loop-mediated isothermal amplification (LAMP): An effective molecular point-of-care technique for the rapid diagnosis of coronavirus SARS-CoV-2.
机构信息
Laboratory of Medical Parasitology, Biotechnology and Biomolecules LR 11 IPT 06, Institut Pasteur de Tunis, Tunis, Tunisia.
Laboratory of Bioinformatics, Biomathematics and Biostatistics LR 16 IPT 09, Institut Pasteur de Tunis, Tunis, Tunisia.
出版信息
Rev Med Virol. 2021 Nov;31(6):e2215. doi: 10.1002/rmv.2215. Epub 2021 Jan 21.
Abstract
The novel coronavirus disease-2019 (Covid-19) public health emergency has caused enormous loss around the world. This pandemic is a concrete example of the existing gap between availability of advanced diagnostics and current need for cost-effective methodology. The advent of the loop-mediated isothermal amplification (LAMP) assay provided an innovative tool for establishing a rapid diagnostic technique based on the molecular amplification of pathogen RNA or DNA. In this review, we explore the applications, diagnostic effectiveness of LAMP test for molecular diagnosis and surveillance of severe acute respiratory syndrome coronavirus 2. Our results show that LAMP can be considered as an effective point-of-care test for the diagnosis of Covid-19 in endemic areas, especially for low- and middle-income countries.
摘要
新型冠状病毒病-2019（COVID-19）公共卫生紧急情况在全球范围内造成了巨大的损失。这场大流行是先进诊断方法的可用性与当前对经济高效方法的需求之间存在差距的具体体现。环介导等温扩增（LAMP）检测的出现为建立基于病原体 RNA 或 DNA 分子扩增的快速诊断技术提供了一种创新工具。在这篇综述中，我们探讨了 LAMP 试验在分子诊断和严重急性呼吸系统综合征冠状病毒 2 监测中的应用和诊断效果。我们的结果表明，LAMP 可以被认为是流行地区 COVID-19 诊断的有效床边检测方法，特别是对于低收入和中等收入国家。
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