Varela de Andrade Anderson, Sartori Pereira Fernando, Nascimento da Silva Fabio, Felippe da Silva Gustavo, de Lourdes Borba Magalhães Maria
Universidade do Estado de Santa Catarina, Lages, SC, Brazil.
Universidade do Estado de Santa Catarina, Lages, SC, Brazil.
J Genet Eng Biotechnol. 2024 Jun;22(2):100373. doi: 10.1016/j.jgeb.2024.100373. Epub 2024 Apr 4.
Wheat stripe mosaic virus (WhSMV) is a significant wheat pathogen that causes substantial yield losses in Brazil and other countries. Although several detection methods are available, reliable and efficient tools for on-site WhSMV detection are currently lacking. In this study, a Loop-Mediated Isothermal Amplification (LAMP) method was developed for rapid and reliable field detection of WhSMV. We designed WhSMV-specific primers for the LAMP assay and optimized reaction conditions for increased sensitivity and specificity using infected plant samples.
We have developed a diagnostic method utilizing the Loop-Mediated Isothermal Amplification (LAMP) technique capable of rapidly and reliably detecting WhSMV. The LAMP assay has been optimized to enhance sensitivity, specificity, and cost-effectiveness.
The LAMP assay described here represents a valuable tool for early WhSMV detection, serving to mitigate the adverse economic and social impacts of this viral pathogen. By enabling swift and accurate identification, this assay can significantly improve the sustainability of cereal production systems, safeguarding crop yields against the detrimental effects of WhSMV.
小麦条纹花叶病毒（WhSMV）是一种重要的小麦病原体，在巴西和其他国家会导致大量产量损失。尽管有几种检测方法可用，但目前缺乏用于现场检测WhSMV的可靠且高效的工具。在本研究中，开发了一种环介导等温扩增（LAMP）方法用于快速、可靠地现场检测WhSMV。我们为LAMP检测设计了WhSMV特异性引物，并使用感染的植物样本优化了反应条件以提高灵敏度和特异性。
我们开发了一种利用环介导等温扩增（LAMP）技术的诊断方法，能够快速、可靠地检测WhSMV。LAMP检测已进行优化以提高灵敏度、特异性和成本效益。
本文所述的LAMP检测是早期检测WhSMV的有价值工具，有助于减轻这种病毒病原体的不利经济和社会影响。通过实现快速准确的鉴定，该检测可显著提高谷物生产系统的可持续性，保护作物产量免受WhSMV的有害影响。
