College of Pharmacy, Chungbuk National University, Cheongju 28160, Korea.
Department of Plant Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea.
J Nat Prod. 2021 Feb 26;84(2):230-238. doi: 10.1021/acs.jnatprod.0c00842. Epub 2021 Jan 21.
Bioactivity-guided isolation of a MeOH extract of led to the isolation of four new -pimarane diterpenoids (-) and a diacetylene () together with 21 known compounds (-). Their structures were established based on the interpretation of one- and two-dimensional NMR and HRESIMS data. The absolute configurations of the new isolates were determined by electronic circular dichroism data analysis, single crystal X-ray diffraction, and Mosher's esterification method. All compounds exhibited inhibitory effects on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophages with IC values ranging from 1.1 to 69.4 μM.
采用生物活性导向分离法，从 的甲醇提取物中分离得到了四个新的 -松烷二萜 (-) 和一个二炔 ()，以及 21 个已知化合物 (-)。根据一维和二维 NMR 及 HRESIMS 数据的解析，确定了它们的结构。新分离物的绝对构型通过电子圆二色性数据分析、单晶 X 射线衍射和 Mosher 酯化法确定。所有化合物均表现出对 LPS 诱导的 RAW 264.7 巨噬细胞中一氧化氮生成的抑制作用，IC 值范围为 1.1 至 69.4 μM。
