Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan, Guangdong, 528225, China.
Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan, Guangdong, 528225, China.
Dev Comp Immunol. 2021 Jun;119:104018. doi: 10.1016/j.dci.2021.104018. Epub 2021 Jan 18.
Edwardsiella tarda, the bacterial pathogen that causes ascites disease and red-head disease, poses a serious threat to yellow catfish (Pelteobagrus fulvidraco) aquaculture. In this study, the spleens of E. tarda-infected and non-infected yellow catfish were sequenced to obtain the microRNA (miRNA) and mRNA expression profiles. We obtained 657 differentially expressed (DE) miRNAs and 6867 DE mRNAs between two groups and annotated them using the KEGG database. In addition, the 43 negatively correlated miRNA-mRNA pairs were identified using integrated miRNA-mRNA analysis, which including immune-related miRNAs and target genes such as miR-144, miR-1260, miR-1388, miR-33, miR-338, miR-181b, miR-34c, miR-135 and CLEC4E, LITR, PIKfyve, NCF4, IL-12β, IP6K2, TNFRSF9, IL-4Rα, IRF2, Mx2. We verified 8 DE miRNAs pairs and 10 DE mRNAs by quantitative real-time PCR. Finally, the CLEC4E and Mx2 mRNAs were selected for further verification using in situ hybridization. Together, our results provide valuable information for further analyses of the mechanisms of yellow catfish defense against E. tarda infection.
迟缓爱德华氏菌是引起腹水病和红头病的病原菌，对黄颡鱼（Pelteobagrus fulvidraco）养殖业构成严重威胁。本研究对迟缓爱德华氏菌感染和未感染黄颡鱼的脾脏进行测序，获得了 microRNA（miRNA）和 mRNA 表达谱。我们在两组之间获得了 657 个差异表达（DE）miRNA 和 6867 个 DE mRNAs，并使用 KEGG 数据库对其进行了注释。此外，通过整合 miRNA-mRNA 分析鉴定了 43 对负相关的 miRNA-mRNA 对，其中包括免疫相关的 miRNA 和靶基因，如 miR-144、miR-1260、miR-1388、miR-33、miR-338、miR-181b、miR-34c、miR-135 和 CLEC4E、LITR、PIKfyve、NCF4、IL-12β、IP6K2、TNFRSF9、IL-4Rα、IRF2、Mx2。我们通过定量实时 PCR 验证了 8 个 DE miRNA 对和 10 个 DE mRNAs。最后，选择 CLEC4E 和 Mx2 mRNA 进行原位杂交进一步验证。总之，我们的结果为进一步分析黄颡鱼防御迟缓爱德华氏菌感染的机制提供了有价值的信息。
