College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, PR China.
College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China; Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing 100193, China.
Bioresour Technol. 2021 Apr;325:124703. doi: 10.1016/j.biortech.2021.124703. Epub 2021 Jan 15.
The purpose of this study was to investigate fungal diversity and relative abundance (RA) during pig manure composting via high-throughput sequencing approach. Fine coal gasification slag (FCGS) (0%, 2%, 4%, 6%, 8% and 10%) were added into composting raw materials as additive and performed 42 days. Adjust C/N and moisture to 30 and 65%. Results showed that dominant phyla were Ascomycota (99.62%) and Basidiomycota (0.38%). The main genera were Epicoccum (1.26%), Alternaria (83.35%), Aspergillus (12.08%) and Gibberella (1.69%). 10% treatment got the higher abundance and operational taxonomic units number from rank abundance curve and petals diagram. Compared with control, FCGS amendment composting could increase the sanitary time (3-7 d) and total nitrogen (0.05-12.03%). The principal component analysis was considered that FCGS treatments and control had significantly difference. The RA of fungi varied among all treatments. Therefore, 10% treatment was a potential candidate to enhance fungal diversity and composting quality.
本研究旨在通过高通量测序方法研究猪粪堆肥过程中的真菌多样性和相对丰度（RA）。将细煤气化渣（FCGS）（0%、2%、4%、6%、8%和 10%）添加到堆肥原料中作为添加剂，并进行 42 天的处理。调整 C/N 和水分至 30%和 65%。结果表明，优势门为子囊菌门（99.62%）和担子菌门（0.38%）。主要属为层出镰刀菌（1.26%）、链格孢菌（83.35%）、曲霉属（12.08%）和赤霉属（1.69%）。从等级丰度曲线和花瓣图可以看出，10%处理的丰度和操作分类单元数更高。与对照组相比，FCGS 改良堆肥可以缩短卫生时间（3-7d）并增加总氮（0.05-12.03%）。主成分分析认为 FCGS 处理组和对照组有显著差异。真菌的 RA 在所有处理中均有差异。因此，10%的处理是增强真菌多样性和堆肥质量的潜在候选方案。
