过氧化物酶 CCBAS 930 生物转化蒽环类抗生素及其降低遗传毒性、细胞毒性和促氧化活性的可能性。
Possibility to Biotransform Anthracyclines by Peroxidases Produced by CCBAS 930 with Reduction of Geno- and Cytotoxicity and Pro-Oxidative Activity.
机构信息
Department of Environmental Microbiology, Laboratory of Mycology, The University of Life Sciences, Leszczyńskiego Street 7, 20-069 Lublin, Poland.
Department of Lifestyle Disorders and Regenerative Medicine, University of Information Technology and Management in Rzeszow, Sucharskiego Street 2, 35-225 Rzeszow, Poland.
出版信息
Molecules. 2021 Jan 17;26(2):462. doi: 10.3390/molecules26020462.
The aim of this study was to evaluate the bioremoval mechanism of anthracycline antibiotics by the white-rot fungus CCBAS 930. The activity of oxidoreductases and levels of phenolic compounds and free radicals were determined during the biotransformation of anthraquinone antibiotics: daunomycin (DNR) and doxorubicin (DOX) by strain CCBAS 930. Moreover, phytotoxicity ( L.), ecotoxicity (), genotoxicity and cytotoxicity of anthraquinone dyes were evaluated before and after biological treatment. More than 80% and 90% of DNR and DOX were removed by biodegradation (decolorization). Initial solutions of DNR and DOX were characterized by eco-, phyto-, geno- and cytotoxicity. Despite efficient decolorization, secondary metabolites, toxic to bacteria, formed during biotransformation of anthracycline antibiotics in CCBAS 930 cultures. DNR and DOX metabolites did not increase reactive oxygen species (ROS) production in human fibroblasts and resazurin reduction. DNR metabolites did not change caspase-3 activity.
本研究旨在评估白腐真菌 CCBAS 930 对蒽环类抗生素的生物去除机制。在菌株 CCBAS 930 生物转化蒽醌类抗生素：柔红霉素（DNR）和多柔比星（DOX）的过程中，测定了氧化还原酶的活性、酚类化合物和自由基的水平。此外，在生物处理前后还评估了蒽醌染料的植物毒性（ L.）、生态毒性（）、遗传毒性和细胞毒性。生物降解（脱色）可去除超过 80%和 90%的 DNR 和 DOX。DNR 和 DOX 的初始溶液具有生态毒性、植物毒性、遗传毒性和细胞毒性。尽管脱色效果显著，但在 CCBAS 930 培养物中进行蒽环类抗生素的生物转化过程中会形成对细菌有毒的次级代谢物。DNR 和 DOX 的代谢物不会增加人成纤维细胞中的活性氧（ROS）产生和 Resazurin 还原。DNR 代谢物不会改变 caspase-3 活性。
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