Guardiola-Avila Iliana, Sánchez-Busó Leonor, Acedo-Félix Evelia, Gomez-Gil Bruno, Zúñiga-Cabrera Manuel, González-Candelas Fernando, Noriega-Orozco Lorena
Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD), Hermosillo, Sonora 83304, Mexico.
Genomics and Health Area, Foundation for the Promotion of Health and Biomedical Research in the Valencian Community (FISABIO-Public Health), 46020 Valencia, Spain.
Microorganisms. 2021 Jan 18;9(1):191. doi: 10.3390/microorganisms9010191.
is an emerging pathogen, mainly associated with contaminated seafood consumption. However, little is known about its evolution, biodiversity, and pathogenic potential. This study analyzes the pan-, core, and accessory genomes of nine strains. The core genome yielded 2424 genes in chromosome I (ChI) and 822 genes in chromosome II (ChII), with an accessory genome comprising an average of 10.9% of the whole genome for ChI and 29% for ChII. Core genome phylogenetic trees were obtained, and ATCC-33654 strain was the closest to the outgroup in both chromosomes. Additionally, a phylogenetic study of eight conserved genes (Z, A, B, , A, A, B, and H), including , , , and , clearly showed clade differentiation. The main virulence genes found in ChI corresponded with type I secretion proteins, extracellular components, flagellar proteins, and potential regulators, while, in ChII, the main categories were type-I secretion proteins, chemotaxis proteins, and antibiotic resistance proteins. The accessory genome was characterized by the presence of mobile elements and toxin encoding genes in both chromosomes. Based on the genome atlas, it was possible to characterize differential regions between strains. The pan-genome of encompassed 3539 genes for ChI and 2355 genes for ChII. These results give us an insight into the virulence and gene content of , as well as constitute the first approach to its diversity.
是一种新兴病原体，主要与食用受污染的海鲜有关。然而，对其进化、生物多样性和致病潜力知之甚少。本研究分析了9株菌株的泛基因组、核心基因组和辅助基因组。核心基因组在染色体I（ChI）中产生2424个基因，在染色体II（ChII）中产生822个基因，辅助基因组在ChI中平均占全基因组的10.9%，在ChII中占29%。获得了核心基因组系统发育树，ATCC - 33654菌株在两条染色体上都最接近外群。此外，对包括 、 、 、 和 在内的8个保守基因（Z、A、B、 、A、A、B和H）的系统发育研究清楚地显示了进化枝分化。在ChI中发现的主要毒力基因与I型分泌蛋白、细胞外成分、鞭毛蛋白和潜在调节因子相对应，而在ChII中，主要类别是I型分泌蛋白、趋化蛋白和抗生素抗性蛋白。辅助基因组的特征是两条染色体上都存在移动元件和毒素编码基因。基于基因组图谱，可以表征菌株之间的差异区域。 的泛基因组在ChI中包含3539个基因，在ChII中包含2355个基因。这些结果让我们深入了解了 的毒力和基因含量，同时也构成了对其多样性的首次研究。
