Zhen Yuanhang, Zhang Tao, Jiang Bo, Chen Jingjing
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China.
Foods. 2021 Jan 18;10(1):185. doi: 10.3390/foods10010185.
In this study, an efficient method for the purification of resistant dextrin (RD) using membrane filtration and anion exchange resin decolorization was developed, then the purified RD was characterized. In the membrane filtration stage, suspended solids in RD were completely removed, and the resulting product had a negligible turbidity of 2.70 ± 0.18 NTU. Furthermore, approximately half of the pigments were removed. Static decolorization experiments revealed that the D285 anion exchange resin exhibited the best decolorization ratio (D%), 84.5 ± 2.03%, and recovery ratio (R%), 82.8 ± 1.41%, among all the tested resins. Under optimal dynamic decolorization conditions, the D% and R% of RD were 86.26 ± 0.63% and 85.23 ± 0.42%, respectively. The decolorization efficiency of the D285 resin was superior to those of activated carbon and HO. Moreover, the chemical characteristics and molecular weight of RD did not change significantly after purification. The nuclear magnetic resonance spectroscopy of RD showed the formation of new glycosidic linkages that are resistant to digestive enzymes. The superior water solubility (99.14%), thermal stability (up to 200 °C), and rheological properties of RD make it possible to be widely used in food industry.
在本研究中，开发了一种使用膜过滤和阴离子交换树脂脱色来纯化抗性糊精（RD）的有效方法，然后对纯化后的RD进行了表征。在膜过滤阶段，RD中的悬浮固体被完全去除，所得产品的浊度可忽略不计，为2.70±0.18 NTU。此外，约一半的色素被去除。静态脱色实验表明，在所有测试树脂中，D285阴离子交换树脂表现出最佳的脱色率（D%），为84.5±2.03%，回收率（R%）为82.8±1.41%。在最佳动态脱色条件下，RD的D%和R%分别为86.26±0.63%和85.23±0.42%。D285树脂的脱色效率优于活性炭和HO。此外，纯化后RD的化学特性和分子量没有明显变化。RD的核磁共振光谱显示形成了对消化酶具有抗性的新糖苷键。RD优异的水溶性（99.14%）、热稳定性（高达200℃）和流变学性质使其有可能在食品工业中广泛应用。
