Chen Xusheng, Li Qin, He Honggang, Zhang Jianhua, Mao Zhonggui
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University 1800 Lihu Road Wuxi 214122 Jiangsu China
RSC Adv. 2019 Apr 17;9(21):12174-12181. doi: 10.1039/c9ra00493a. eCollection 2019 Apr 12.
ε-Poly-l-lysine (ε-PL) is an added-value natural product with widespread application in the fields of food, pharmaceuticals and biopolymer materials. However, the high production cost reduces its application. To improve the efficiency of ε-PL purification for decreasing the cost of downstream processes, the ion form of the ion-exchange resin, which is widely used for ε-PL purification, was investigated systematically in this study. Among eleven cation-exchange resins tested, the Amberlite IRC-50 resin offered the best adsorption capability and the highest desorption ratio. The adsorption kinetics of IRC-50 resin with H, Na and NH ion forms followed a pseudo-second-order model. The dynamic adsorption and desorption parameters of ε-PL were optimized with a column packed with IRC-50 resin with Na and NH . It is suggested that NH is the optimal ion form of IRC-50 resin for ε-PL extraction. Under optimal conditions, the IRC-50 resin with NH achieved the highest ε-PL adsorption capability, purity and recovery ratio of 307.96 mg g, 76.52% and 96.2%, respectively. After further purification, a final ε-PL purity of 97.10% was achieved with a total recovery ratio of 66.01%. This is the first report on improving the ε-PL purification efficiency through optimizing the ion form of the ion-exchange resin. Moreover, it would offer guidance for other natural product recovery processes by ion-exchange chromatography.
ε-聚-L-赖氨酸（ε-PL）是一种具有广泛应用价值的天然产物，在食品、制药和生物高分子材料等领域有着广泛应用。然而，其高昂的生产成本限制了它的应用。为了提高ε-PL的纯化效率以降低下游工艺成本，本研究系统地考察了广泛用于ε-PL纯化的离子交换树脂的离子形式。在所测试的11种阳离子交换树脂中，Amberlite IRC-50树脂表现出最佳的吸附能力和解吸率。IRC-50树脂在H、Na和NH离子形式下的吸附动力学符合准二级模型。用填充有Na和NH离子形式的IRC-50树脂的柱子对ε-PL的动态吸附和解吸参数进行了优化。结果表明，NH是IRC-50树脂用于ε-PL提取的最佳离子形式。在最佳条件下，NH离子形式的IRC-50树脂对ε-PL的吸附能力、纯度和回收率分别达到307.96 mg/g, 76.52%和96.2%。经过进一步纯化，最终ε-PL的纯度达到97.10%，总回收率为66.01%。这是首次通过优化离子交换树脂的离子形式来提高ε-PL纯化效率的报道。此外，它将为离子交换色谱法回收其他天然产物的工艺提供指导。
