Xiong Qingping, Zhang Qianghua, Zhang Danyan, Shi Yingying, Jiang Changxing, Shi Xiaojuan
College of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
Food Chem. 2014 Feb 15;145:1-7. doi: 10.1016/j.foodchem.2013.07.140. Epub 2013 Aug 9.
In the present study, the separation and purification characteristics of resveratrol from extract of peanut sprouts (PSE) on 11 macroporous adsorption resins were investigated. The results showed that ADS-5 offered better adsorption and desorption capacity for resveratrol than other tested resins. From the static experiments with ADS-5 resin, we found that the experimental data fitted best to the pseudo-second-order kinetics model and Langmuir isotherm model, and the adsorption was a spontaneous and endothermic process. The separation and purification parameters of resveratrol from PSE were optimised by dynamic adsorption/desorption experiments with the column packed with ADS-5 resin. Under the optimal conditions, after one run treatment with ADS-5 resin, the content of resveratrol in the product was increased 17.9-fold from 1.32% to 23.60%, with a recovery yield of 88.33%. The results demonstrated that ADS-5 resin was a promising basis for large-scale preliminary separation and purification of resveratrol from PSE.
在本研究中,考察了花生芽提取物(PSE)中白藜芦醇在11种大孔吸附树脂上的分离纯化特性。结果表明,ADS - 5对白藜芦醇的吸附和解吸能力优于其他受试树脂。通过ADS - 5树脂的静态实验,我们发现实验数据最符合准二级动力学模型和朗缪尔等温线模型,且吸附是一个自发的吸热过程。采用填充ADS - 5树脂的色谱柱通过动态吸附/解吸实验对白藜芦醇从PSE中的分离纯化参数进行了优化。在最佳条件下,用ADS - 5树脂进行一次运行处理后,产物中白藜芦醇的含量从1.32%提高到23.60%,提高了17.9倍,回收率为88.33%。结果表明,ADS - 5树脂是从PSE中大规模初步分离纯化白藜芦醇的有前景的基础材料。