Wysokińska Anna, Wójcik Ewa, Chłopik Angelika
Faculty of Agrobioengineering and Animal Husbandry, Siedlce University of Natural Sciences and Humanities, 08110 Siedlce, Poland.
Animals (Basel). 2021 Jan 18;11(1):227. doi: 10.3390/ani11010227.
Evaluation of sperm morphometry is an important criterion in the diagnosis of a male animal's suitability for breeding. The aim of the study was to evaluate the morphometry of sperm from the epididymides of dogs subjected to routine castration using various staining methods. The study was carried out on semen collected from ten healthy dogs. Gonads were obtained from each dog during routine castration at a veterinary surgery. Then, the epididymides (caput, corpus, cauda) were isolated from the gonads, semen was collected from them and microscope slides were prepared. The slides for evaluation of sperm morphometry were prepared by four methods: DiffQuik, SpermBlue, eosin-nigrosin and eosin-gentian. A total of 2400 sperm were analyzed (240 sperm from the dog). The sperm collected from the caput and corpus of the epididymis were found to have larger heads and tails than those collected from the cauda of the epididymis. The staining method was shown to affect the morphometry of sperm taken from the epididymides of dogs. The staining methods differentiate the dimensions of the head of sperm in different parts of the epididymis but do not affect the length of the sperm tail. The occurrence of differences in the head dimensions of sperm may be linked to the use of different fixatives and chemical reagents in the staining procedure. Sperm stained by the EN method had the smallest head and tail dimensions. The greatest head area was noted in the sperm stained by the EG method. In the slides stained by the SB method, the sperm heads were relatively long but narrow. The methods used are suitable for the evaluation of sperm structure, and the possibility of using all four methods enables a full characterization of sperm collected from the caput, corpus and cauda of the epididymides of dogs.
评估精子形态测量是诊断雄性动物繁殖适宜性的一项重要标准。本研究的目的是使用各种染色方法评估接受常规阉割的犬附睾精子的形态测量。该研究对从十只健康犬采集的精液进行。在兽医手术的常规阉割过程中从每只犬获取性腺。然后，从性腺中分离出附睾（头、体、尾），从附睾中采集精液并制备显微镜载玻片。用于评估精子形态测量的载玻片通过四种方法制备：DiffQuik、SpermBlue、伊红 - 黑色素和伊红 - 龙胆紫。总共分析了2400个精子（每只犬240个精子）。发现从附睾头和体采集的精子头部和尾部比从附睾尾采集的精子更大。结果表明染色方法会影响从犬附睾采集的精子的形态测量。染色方法可区分附睾不同部位精子头部的尺寸，但不影响精子尾部的长度。精子头部尺寸出现差异可能与染色过程中使用不同的固定剂和化学试剂有关。用伊红 - 黑色素方法染色的精子头部和尾部尺寸最小。用伊红 - 龙胆紫方法染色的精子头部面积最大。在SpermBlue方法染色的载玻片中，精子头部相对较长但较窄。所使用的方法适用于评估精子结构，并且使用所有四种方法的可能性能够全面表征从犬附睾头、体和尾采集的精子。
