Department of Animal Reproduction - School of Veterinary Medicine and Animal Science, University of São Paulo, campus São Paulo, São Paulo, Brazil.
Anim Biotechnol. 2021 Feb;32(1):92-99. doi: 10.1080/10495398.2019.1657126. Epub 2019 Aug 22.
In order to add information to physiology of sperm maturation and help to underline future antioxidant supplementation treatment to epididymal sperm, the aim of this study was to evaluate susceptibility of caput, corpus and cauda epididymal sperm to different reactive oxygen species (ROS) in dogs. Epididymal sperm was separately collected from each segment (caput, corpus and cauda) and subjected to 4 different ROS-challenges: superoxide anion, hydrogen peroxide, hydroxyl radical, malondialdehyde (MDA) or maintained as control. After 30 min of incubation in each ROS, sperm was evaluated for sperm kinetics, plasma and acrossomal membrane integrity, mitochondrial activity and resistance to oxidative stress. Decreased total and progressive sperm motility and rapid velocity at epididymal corpus occurred after exposure to hydrogen peroxide, hydroxyl radical and MDA. However, for cauda epididymis, hydrogen peroxide and malondialdehyde promoted higher deleterious effect regarding sperm motility and velocity. Only at cauda epididymis MDA decreased sperm mitochondrial activity index and no kinetics alterations (motility or velocity) occurred after exposure to superoxide anion in corpus and cauda epididymis. In conclusion, corpus and cauda epididymal sperm are highly susceptible to deleterious effect of hydrogen peroxide, malondialdehyde and hydroxyl radical. In addition, epididymal canine sperm is relatively resistant to superoxide anion damage.
为了增加精子成熟生理学方面的信息，并有助于强调未来对附睾精子的抗氧化补充治疗，本研究旨在评估犬附睾头部、体部和尾部精子对不同活性氧（ROS）的敏感性。从每个区域（头部、体部和尾部）分别收集附睾精子，并对其进行 4 种不同的 ROS 挑战：超氧阴离子、过氧化氢、羟自由基、丙二醛（MDA）或保持对照。在每种 ROS 孵育 30 分钟后，评估精子的动力学、血浆和跨膜完整性、线粒体活性和抗氧化应激能力。过氧化氢、羟自由基和 MDA 暴露后，附睾体部的总精子和前向运动精子活力以及快速速度降低。然而，对于附睾尾部，过氧化氢和丙二醛对精子活力和速度的有害影响更大。只有在附睾尾部，MDA 降低了精子线粒体活性指数，而在体部和尾部附睾中，超氧阴离子暴露后没有出现动力学改变（活力或速度）。总之，附睾体部和尾部精子对过氧化氢、丙二醛和羟自由基的有害作用非常敏感。此外，犬附睾精子对超氧阴离子损伤具有相对抗性。
