Du Xin, Yu Lei, Yang Ling, Cao Dingfang, Zhang Ying
Department of Thoracic Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
Central Laboratory, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
Zhongguo Fei Ai Za Zhi. 2021 Jan 20;24(1):1-6. doi: 10.3779/j.issn.1009-3419.2021.102.03.
Thymomas are the most common primary malignant tumors of anterior mediastinal. However, there are no specific laboratory indicator for the diagnosis the diagnosis of thymoma. The aim of this study was to screen out a tumor marker for diagnosis of thymoma by mRNA microarray analysis and confirmed it.
By mRNA microarray analysis of 31 thymomas and peritumoral thymic tissues, we found that the transcription level of neuronal pentraxin 1 (NPTX1) gene was up-regulated more than 4 times in thymomas. To further verify the above results, we detected the transcription and expression level of NPTX1 in 60 thymoma and 30 thymic cyst patients by quantitative Real-Time polymerase chain reaction (PCR), immunohistochemistry and enzyme-linked immunosorbent assay (ELISA). Furthermore, the diagnostic value of NPTX1 in thymoma by receiver operating characteristic curve (ROC) was analyzed.
The transcription level of NPTX1 mRNA in thymoma tissues was significantly higher than that in the thymic tissues of control group [(2.88±1.02) vs (1.35±0.47), P<0.001); The expression level of NPTX1 in thymoma tissues was significantly higher than that in the thymic tissues of control group (2 vs 1, P<0.001); The preoperative serum level of NPTX1 protein in thymoma patients were significantly higher than that in the thymic cyst patients of control group [(1,018.29±209.38) pg/mL vs (759.95±66.02) pg/mL, P<0.001]; At the threshold of 842.22 pg/mL, sensitivity and specificity of NPTX1 as a serologic marker were 85.00% and 93.33%, respectively for thymoma. ROC showed that the area the under curve (AUC) of NPTX1 was 0.902.
NPTX1 was highly expressed in thymoma patients, and had diagnostic value for thymoma.
胸腺瘤是前纵隔最常见的原发性恶性肿瘤。然而，目前尚无用于诊断胸腺瘤的特异性实验室指标。本研究旨在通过mRNA芯片分析筛选出一种用于胸腺瘤诊断的肿瘤标志物并加以验证。
通过对31例胸腺瘤及瘤旁胸腺组织进行mRNA芯片分析，我们发现神经元五聚体蛋白1（NPTX1）基因在胸腺瘤中的转录水平上调超过4倍。为进一步验证上述结果，我们采用定量实时聚合酶链反应（PCR）、免疫组织化学和酶联免疫吸附测定（ELISA）检测了60例胸腺瘤患者和30例胸腺囊肿患者中NPTX1的转录和表达水平。此外，通过绘制受试者工作特征曲线（ROC）分析了NPTX1对胸腺瘤的诊断价值。
胸腺瘤组织中NPTX1 mRNA的转录水平显著高于对照组胸腺组织[（2.88±1.02）对（1.35±0.47），P<0.001]；胸腺瘤组织中NPTX1的表达水平显著高于对照组胸腺组织（2对1，P<0.001）；胸腺瘤患者术前血清NPTX1蛋白水平显著高于对照组胸腺囊肿患者[（1,018.29±209.38）pg/mL对（759.95±66.02）pg/mL，P<0.001]；在阈值为842.22 pg/mL时，NPTX1作为胸腺瘤血清学标志物的敏感性和特异性分别为85.00%和93.33%。ROC曲线显示NPTX1的曲线下面积（AUC）为0.902。
NPTX1在胸腺瘤患者中高表达，对胸腺瘤具有诊断价值。
