Miller R H
Hepatitis Viruses Section, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
Science. 1988 Mar 18;239(4846):1420-2. doi: 10.1126/science.3347840.
The genome of the human immunodeficiency virus (HIV) is known to contain eight open reading frames (ORFs) on the minus strand of the double-stranded DNA replicative intermediate. Data presented here indicate that the DNA plus strand of HIV contains a previously unidentified ORF in a region complementary to the envelope gene sequence. This ORF could encode a protein of approximately 190 amino acid residues with a relative molecular mass of 20 kilodaltons if translation began from the first initiation codon. The predicted protein is highly hydrophobic and thus could be membrane associated. It is possible, therefore, that the HIV genome encodes a protein on antisense messenger RNA.
已知人类免疫缺陷病毒（HIV）的基因组在双链DNA复制中间体的负链上包含八个开放阅读框（ORF）。此处呈现的数据表明，HIV的DNA正链在与包膜基因序列互补的区域含有一个先前未鉴定的ORF。如果从第一个起始密码子开始翻译，这个ORF可能编码一个约190个氨基酸残基、相对分子质量为20千道尔顿的蛋白质。预测的蛋白质具有高度疏水性，因此可能与膜相关。因此，HIV基因组有可能在反义信使RNA上编码一种蛋白质。
