热休克蛋白 22（HSPB8）正向调节 PGF2α 诱导的成骨细胞中白细胞介素 6 和血管内皮生长因子的合成。
HSP22 (HSPB8) positively regulates PGF2α-induced synthesis of interleukin-6 and vascular endothelial growth factor in osteoblasts.
机构信息
Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Micuho-cho, Mizuho-ku, Nagoya, 467-8601, Japan.
Department of Rehabilitation Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya, 467-8601, Japan.
出版信息
J Orthop Surg Res. 2021 Jan 21;16(1):72. doi: 10.1186/s13018-021-02209-8.
BACKGROUND
Heat shock protein 22 (HSP22) belongs to class I of the small HSP family that displays ubiquitous expression in osteoblasts. We previously demonstrated that prostaglandin F2α (PGF2α), a potent bone remodeling factor, induces the synthesis of interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) via p44/p42 mitogen-activated protein (MAP) kinase and p38 MAP kinase in osteoblast-like MC3T3-E1 cells. In the present study, we investigated whether HSP22 is implicated in the PGF2α-induced synthesis of IL-6 and VEGF and the mechanism of MC3T3-E1 cells.
METHODS
MC3T3-E1 cells were transfected with HSP22-siRNA. IL-6 and VEGF release was assessed by ELISA. Phosphorylation of p44/p42 MAP kinase and p38 MAP kinase was detected by Western blotting.
RESULTS
The PGF2α-induced release of IL-6 in HSP22 knockdown cells was significantly suppressed compared with that in the control cells. HSP22 knockdown also reduced the VEGF release by PGF2α. Phosphorylation of p44/p42 MAP kinase and p38 MAP kinase was attenuated by HSP22 downregulation.
CONCLUSIONS
Our results strongly suggest that HSP22 acts as a positive regulator in the PGF2α-induced synthesis of IL-6 and VEGF in osteoblasts.
背景
热休克蛋白 22（HSP22）属于小热休克蛋白家族 I 类，在成骨细胞中广泛表达。我们之前的研究表明，前列腺素 F2α（PGF2α）作为一种强有力的骨重塑因子，通过成骨样 MC3T3-E1 细胞中的 p44/p42 有丝分裂原激活蛋白（MAP）激酶和 p38 MAP 激酶诱导白细胞介素 6（IL-6）和血管内皮生长因子（VEGF）的合成。在本研究中，我们研究了 HSP22 是否参与 PGF2α 诱导的 IL-6 和 VEGF 合成以及 MC3T3-E1 细胞的机制。
方法
用 HSP22-siRNA 转染 MC3T3-E1 细胞。通过 ELISA 评估 IL-6 和 VEGF 的释放。通过 Western 印迹检测 p44/p42 MAP 激酶和 p38 MAP 激酶的磷酸化。
结果
与对照细胞相比，HSP22 敲低细胞中 PGF2α 诱导的 IL-6 释放明显受到抑制。HSP22 敲低也减少了 PGF2α 诱导的 VEGF 释放。HSP22 下调减弱了 p44/p42 MAP 激酶和 p38 MAP 激酶的磷酸化。
结论
我们的研究结果强烈表明，HSP22 作为成骨细胞中 PGF2α 诱导的 IL-6 和 VEGF 合成的正调节剂发挥作用。
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