Ruminant Diseases and Immunology Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA, USA.
Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
Vet Res. 2021 Jan 21;52(1):11. doi: 10.1186/s13567-021-00893-2.
Infection with Mycobacterium avium subspecies paratuberculosis (MAP) is complex, but little is known about the role that natural killer (NK) cells play. In the present study, four bovine NK-lysin peptides were synthesized to evaluate their bactericidal activity against MAP. The results demonstrated that bNK-lysin peptides were directly bactericidal against MAP, with bNK1 and bNK2A being more potent than bNK2B and bNK2C. Mechanistically, transmission electron microscopy revealed that the incubation of MAP with bNK2A resulted in extensive damage to cell membranes and cytosolic content leakage. Furthermore, the addition of bNK2A linked with a cell-penetrating peptide resulted in increased MAP killing in a macrophage model.
感染鸟分枝杆菌亚种（MAP）的情况很复杂，但目前人们对自然杀伤（NK）细胞所起的作用知之甚少。在本研究中，合成了四种牛 NK 细胞溶素肽，以评估其对 MAP 的杀菌活性。结果表明，牛 NK 细胞溶素肽对 MAP 具有直接杀菌作用，其中 bNK1 和 bNK2A 的杀菌作用强于 bNK2B 和 bNK2C。机制研究显示，MAP 与 bNK2A 孵育后，细胞膜受到广泛损伤，细胞质内容物泄漏。此外，与穿透肽结合的 bNK2A 可增加巨噬细胞模型中 MAP 的杀伤作用。
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