重复 DNA-PAINT 可抑制光学纳米显微镜中的背景和非特异性信号。

Living Systems Institute and Biomedical Physics, University of Exeter, Exeter, EX4 4PY, UK.

Cavendish Laboratory, University of Cambridge, Cambridge, CB3 0HE, UK.

Nat Commun. 2021 Jan 21;12(1):501. doi: 10.1038/s41467-020-20686-z.

DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding of fluorescent DNA 'imagers' to target epitopes. Its performance in biological samples is often constrained by strong background signals and non-specific binding events, both exacerbated by high imager concentrations. Here we describe Repeat DNA-PAINT, a method that enables a substantial reduction in imager concentration, thus suppressing spurious signals. Additionally, Repeat DNA-PAINT reduces photoinduced target-site loss and can accelerate sampling, all without affecting spatial resolution.

DNA-PAINT 是一种多功能的光学超分辨率技术，依赖于荧光 DNA“成像剂”与靶标表位的瞬时结合。在生物样本中的性能通常受到强背景信号和非特异性结合事件的限制，而高浓度的成像剂会加剧这些问题。在这里，我们描述了重复 DNA-PAINT，这是一种可以显著降低成像剂浓度的方法，从而抑制虚假信号。此外，重复 DNA-PAINT 减少了光诱导的靶位点损失，并可以加速采样，所有这些都不会影响空间分辨率。