在优化的 DNA-PAINT 中实现高达 100 倍的速度提升和多重标记。

Up to 100-fold speed-up and multiplexing in optimized DNA-PAINT.

机构信息

Faculty of Physics and Center for Nanoscience, Ludwig Maximilian University, Munich, Germany.

Max Planck Institute of Biochemistry, Martinsried, Germany.

出版信息

Nat Methods. 2020 Aug;17(8):789-791. doi: 10.1038/s41592-020-0869-x. Epub 2020 Jun 29.

DOI:10.1038/s41592-020-0869-x

PMID:32601424

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7610413/

Abstract

DNA-PAINT's imaging speed has recently been significantly enhanced by optimized sequence design and buffer conditions. However, this implementation has not reached an ultimate speed limit and is only applicable to imaging of single targets. To further improve acquisition speed, we introduce concatenated, periodic DNA sequence motifs, yielding up to 100-fold-faster sampling in comparison to traditional DNA-PAINT. We extend this approach to six orthogonal sequence motifs, now enabling speed-optimized multiplexed imaging.

摘要

DNA-PAINT 的成像速度最近通过优化的序列设计和缓冲条件得到了显著提高。然而，这种实现方式尚未达到最终的速度限制，并且仅适用于单个目标的成像。为了进一步提高采集速度，我们引入了串联的周期性 DNA 序列基序，与传统的 DNA-PAINT 相比，采样速度提高了 100 倍。我们将这种方法扩展到六个正交序列基序，现在可以实现速度优化的多路复用成像。