Centre for Forensic Science, Department of Pure and Applied Chemistry, University of Strathclyde, 204 George Street, Glasgow, G1 1XW, Scotland, UK.
Kuwait Identification DNA Laboratory (KIDL), General Department of Criminal Evidence, Ministry of Interior, Kuwait City, Kuwait.
Sci Rep. 2021 Jan 21;11(1):1865. doi: 10.1038/s41598-021-81425-y.
This study evaluates the forensic utility of 23 autosomal short tandem repeat markers in 400 samples from the Kuwaiti population, of which four markers (D10S1248, D22S1045, D2S441 and SE33) are reported for the first time for Kuwait. All the markers were shown to exhibit no deviation from Hardy-Weinberg equilibrium, nor any linkage disequilibrium between and within loci, indicating that these loci are inherited independently, and their allele frequencies can be used to estimate match probabilities in the Kuwaiti population. The low combined match probability of 7.37 × 10 and the high paternity indices generated by these loci demonstrate the usefulness of the PowerPlex Fusion 6C kit for human identification in this population, as well as to strengthen the power of paternity testing. Off-ladder alleles were seen at several loci, and these were identified by examining their underlying nucleotide sequences. Principal component analysis (PCA) and STRUCTURE showed no genetic structure within the Kuwaiti population. However, PCA revealed a correlation between geographic and genetic distance. Finally, phylogenetic trees demonstrated a close relationship between Kuwaitis and Middle Easterners at a global level, and a recent common ancestry for Kuwait with its northern neighbours of Iraq and Iran, at a regional level.
本研究评估了 23 个常染色体短串联重复标记在 400 份来自科威特人群的样本中的法医学实用性，其中四个标记（D10S1248、D22S1045、D2S441 和 SE33）是首次在科威特报道。所有标记均未显示偏离哈迪-温伯格平衡，也未显示在各基因座之间或内部存在连锁不平衡，表明这些基因座是独立遗传的，其等位基因频率可用于估计科威特人群中的匹配概率。这些基因座产生的低联合匹配概率 7.37×10 和高亲子鉴定指数证明了 PowerPlex Fusion 6C 试剂盒在该人群中的人类识别以及增强亲子鉴定能力的有用性。在几个基因座上观察到了离群等位基因，并通过检查其潜在的核苷酸序列来识别这些等位基因。主成分分析（PCA）和 STRUCTURE 显示科威特人群内部没有遗传结构。然而，PCA 显示地理和遗传距离之间存在相关性。最后，系统发育树表明科威特人与中东人在全球范围内密切相关，科威特与北部邻国伊拉克和伊朗在区域层面上也有最近的共同祖先。
