环状 RNA circNINL 通过 miR-921/ADAM9 轴激活 β-连环蛋白信号通路促进乳腺癌进展。

Circular RNA circNINL promotes breast cancer progression through activating β-catenin signaling via miR-921/ADAM9 axis.

机构信息

Department of General Surgery, The Huangdao District People's Hospital, No. 287 Renmin Road, Qingdao, 266400 Shandong, China.

Department of General Surgery, Qingdao Municipal Hospital, No. 5 Middle Donghai Road, Qingdao, 266071 Shandong, China.

出版信息

J Biochem. 2021 Sep 7;169(6):693-700. doi: 10.1093/jb/mvab005.

DOI:10.1093/jb/mvab005

PMID:33479730

Abstract

We investigated the expression and functions of circular RNA (circRNA) circNINL and miR-921 in breast cancer (BC) in this study. We found that the expression of circNINL increased while the expression of miR-921 decreased in BC tissues and cell lines, and their anomalous expressions were associated with malignant features and poor prognostic of BC. Then, we demonstrated that circNINL could interact with miR-921 and facilitate BC cells malignant process including proliferation acceleration, migration enhancement and apoptosis evasion via sponging miR-921 in vitro. Further investigations revealed that circNINL/miR-921 axis could mediate the expression of ADAM9 which was a direct target of miR-921. In addition, we exhibited that ADAM9 may activate β-catenin signaling by interacting with E-cadherin. We presented the vital roles of circNINL/miR-921/ADAM9/β-catenin signaling in the progression of BC.

摘要

在本研究中，我们研究了环状 RNA（circRNA）circNINL 和 miR-921 在乳腺癌（BC）中的表达和功能。我们发现，circNINL 的表达在 BC 组织和细胞系中增加，而 miR-921 的表达减少，它们的异常表达与 BC 的恶性特征和不良预后有关。然后，我们证明 circNINL 可以与 miR-921 相互作用，并通过在体外海绵 miR-921 促进 BC 细胞的恶性进程，包括增殖加速、迁移增强和凋亡逃逸。进一步的研究表明，circNINL/miR-921 轴可以调节 ADAM9 的表达，ADAM9 是 miR-921 的直接靶标。此外，我们发现 ADAM9 可以通过与 E-钙粘蛋白相互作用激活 β-连环蛋白信号通路。我们提出了 circNINL/miR-921/ADAM9/β-连环蛋白信号通路在 BC 进展中的重要作用。