环状RNA NINL促进宫颈癌的恶性进展。

Circular RNA NINL accelerates the malignant progression of cervical cancer.

作者信息

Cao ChengCheng, Zhang HaiFeng, Zhang Ting, Nie CuiCui

机构信息

Department of Gynecology, Affiliated Hospital of Shandong Second Medical University, Shandong, China.

Department of Gynecology and Obstetrics, Sunshine Union Hospital, No. 9000, Yingqian Street, High-Tech Zone, Weifang, 261000, Shandong, China.

出版信息

Discov Oncol. 2024 Dec 18;15(1):766. doi: 10.1007/s12672-024-01588-8.

DOI:10.1007/s12672-024-01588-8

PMID:39692846

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11655911/

Abstract

OBJECTIVE

The study's aim was to explore a novel miRNA/mRNA network mediated by circNINL in cervical cancer (CC).

METHODS

Tumor tissue specimens and normal tissue specimens of 86 CC patients were collected. CircNINL, miR-2467-3p, and specificity protein 1 (SP1) expression levels in tissues were detected. In Hela cells, transfection was implemented to determine whether CircNINL, miR-2467-3p and SP1 regulated cellular progression. The interaction between circNINL, miR-2467-3p, and SP1 was explored.

RESULTS

CircNINL and SP1 were abnormally increased whereas miR-2467-3p expression was suppressed in CC. Functionally, circNINL silencing or miR-2467-3p overexpression reduced CC cell progression, whereas circNINL overexpression did the opposite. CircNINL had a spongy effect on miR-2467-3p to regulate SP1 levels. miR-2467-3p inhibition or SP1 overexpression offset the inhibitory effect of circNINL silence on CC malignant progression.

CONCLUSION

CircNINL silencing can increase miR-2467-3p, thereby inducing the downregulation of SP1, thereby suppressing CC progression.

摘要

目的

本研究旨在探索环状核仁蛋白样蛋白（circNINL）介导的宫颈癌（CC）中新型的微小RNA（miRNA）/信使核糖核酸（mRNA）网络。

方法

收集86例CC患者的肿瘤组织标本和正常组织标本。检测组织中circNINL、miR-2467-3p和特异性蛋白1（SP1）的表达水平。在人宫颈癌HeLa细胞中进行转染，以确定circNINL、miR-2467-3p和SP1是否调节细胞进程。探索circNINL、miR-2467-3p和SP1之间的相互作用。

结果

在CC中，circNINL和SP1异常升高，而miR-2467-3p表达受到抑制。在功能上，circNINL沉默或miR-2467-3p过表达可降低CC细胞进程，而circNINL过表达则产生相反的效果。circNINL对miR-2467-3p具有海绵效应，以调节SP1水平。miR-2467-3p抑制或SP1过表达抵消了circNINL沉默对CC恶性进程的抑制作用。