Reunova G D, Trubitsyn A G, Reifman V G
Institute of Biology and Pedology, Far East Department, USSR Academy of Sciences, Vladivostok.
Virology. 1988 Mar;163(1):198-200. doi: 10.1016/0042-6822(88)90247-4.
The effect on Tobacco mosaic virus (TMV) accumulation of actinomycin D (AMD) introduced shortly after inoculation into isolated tobacco protoplasts under varying light conditions was examined. The emission spectrum of the light source contained lines in the visible range and in the ultraviolet band (300-400 nm). AMD absorbed light in the visible (400-500 nm) and in the uv (200-400 nm) ranges. AMD substantially inhibited TMV multiplication in the light, and also when the protoplasts were incubated in petri dishes covered with a black filter that allowed only uv light in the range 260-390 nm to pass. In the dark, and also in petri dishes covered with blue or yellow filters that passed in the ranges 400-500 or 500-600 nm, respectively, AMD stimulated TMV reproduction. The suppression of TMV multiplication in isolated tobacco protoplasts was assumed to be associated with a photodynamic effect caused by absorption of uv light by AMD.
研究了在不同光照条件下，接种后不久将放线菌素D（AMD）引入分离的烟草原生质体中对烟草花叶病毒（TMV）积累的影响。光源的发射光谱在可见光范围和紫外波段（300 - 400纳米）包含谱线。AMD在可见光（400 - 500纳米）和紫外（200 - 400纳米）范围内吸收光。AMD在光照下以及当原生质体在覆盖有黑色滤光片的培养皿中培养时（该滤光片仅允许260 - 390纳米范围内的紫外光通过），能显著抑制TMV繁殖。在黑暗中，以及在分别覆盖有透过400 - 500纳米或500 - 600纳米范围光的蓝色或黄色滤光片的培养皿中，AMD能刺激TMV繁殖。推测在分离的烟草原生质体中TMV繁殖的抑制与AMD吸收紫外光引起的光动力效应有关。
