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评估牛至油、可水解单宁和茶皂素缓解反刍动物甲烷排放的效果:体内和体外方法。

Evaluation of origanum oil, hydrolysable tannins and tea saponin in mitigating ruminant methane: In vitro and in vivo methods.

机构信息

The Branch Academy of Animal Science, Jilin Academy of Agricultural Sciences, Gongzhuling, China.

Key Laboratory of Beef Cattle Genetics and Breeding, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Gongzhuling, China.

出版信息

J Anim Physiol Anim Nutr (Berl). 2021 Jul;105(4):630-638. doi: 10.1111/jpn.13501. Epub 2021 Jan 21.

DOI:10.1111/jpn.13501
PMID:33480135
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8359363/
Abstract

The objective of this study was to investigate the effects of origanum oil (ORO), hydrolysable tannins (HYT) and tea saponin (TES) on methane (CH ) emission, rumen fermentation, productive performance and gas exchange in sheep by using in vitro and in vivo methods. The ORO, HYT and TES additive levels were normalized per kg dry matter (DM) in both in vitro and in vivo experiments: ORO-0, 10, 20 and 40 ml/kg; HYT-0, 15, 30 and 60 g/kg; and TES-0, 15, 30 and 60 g/kg, respectively. During in vitro incubation, 40 ml/kg ORO linearly decreased CH emission (p < 0.05); 20 and 40 ml/kg ORO cubically decreased carbon dioxide (CO ) production (p < 0.05), and rumen pH was cubically raised with the increasing ORO additive level (p < 0.01). The 60 g/kg HYT cubically decreased CH production (p < 0.05). The pH of 60 g/kg HYT was higher than that of 15 and 30 g/kg (p < 0.01); the pH of 20 g/kg TES was higher than that of 5 g/kg (p < 0.05). In the in vivo experiments, 40 ml/kg ORO inhibited dry matter intake (p < 0.01) cubically and reduced average daily gain (ADG) and feed conversion ratio (FCR) cubically (p < 0.05), and 20 or 40 ml/kg ORO linearly decreased CH production based on per day or metabolic weight (W ) (p < 0.05). Both 30 and 60 g/kg HYT linearly inhibited CH emission on the bases of per day and W (p < 0.05). The 20 g/kg TES improved the apparent digestibility of crude protein (p < 0.05), 10 and 20 g/kg of TES decreased CH emission (p < 0.05), and 5 g/kg of TES reduced O consumption and CO production (p < 0.05). In conclusion, these three plant extracts all showed the abilities on mitigating CH emission of sheep with appropriate additive ranges.

摘要

本研究旨在通过体内和体外方法研究牛至油(ORO)、可水解单宁(HYT)和茶皂素(TES)对绵羊甲烷(CH )排放、瘤胃发酵、生产性能和气体交换的影响。ORO、HYT 和 TES 的添加水平在体内和体外实验中均按每公斤干物质(DM)归一化:ORO-0、10、20 和 40ml/kg;HYT-0、15、30 和 60g/kg;TES-0、15、30 和 60g/kg。在体外培养过程中,40ml/kg 的 ORO 线性降低 CH 排放(p<0.05);20 和 40ml/kg 的 ORO 立方降低二氧化碳(CO )的产生(p<0.05),随着 ORO 添加水平的增加,瘤胃 pH 呈立方上升(p<0.01)。60g/kg 的 HYT 立方降低 CH 产量(p<0.05)。60g/kg 的 HYT 的 pH 值高于 15g/kg 和 30g/kg(p<0.01);20g/kg 的 TES 的 pH 值高于 5g/kg(p<0.05)。在体内实验中,40ml/kg 的 ORO 立方抑制干物质摄入(p<0.01),并立方降低平均日增重(ADG)和饲料转化率(FCR)(p<0.05),并且 20 或 40ml/kg 的 ORO 线性降低基于每日或代谢体重(W )的 CH 产量(p<0.05)。30g/kg 和 60g/kg 的 HYT 均基于每日和 W 线性抑制 CH 排放(p<0.05)。20g/kg 的 TES 提高了粗蛋白的表观消化率(p<0.05),10g/kg 和 20g/kg 的 TES 降低了 CH 排放(p<0.05),而 5g/kg 的 TES 减少了 O 消耗和 CO 产生(p<0.05)。综上所述,这三种植物提取物在适当的添加范围内均表现出降低绵羊 CH 排放的能力。

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