基因组挖掘揭示 中的多产物倍半萜生物合成基因簇
Genome Mining Reveals a Multiproduct Sesterterpenoid Biosynthetic Gene Cluster in .
机构信息
Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, People's Republic of China.
Institute of TCM and Natural Products, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, People's Republic of China.
出版信息
Org Lett. 2021 Mar 5;23(5):1525-1529. doi: 10.1021/acs.orglett.0c03996. Epub 2021 Jan 22.
Genome mining of 094102 enabled the discovery of a multiproduct bifunctional terpene synthase (BTS), AuAS. Heterologous expression of AuAS led to the discovery of five new sesterterpenes, and coexpression of the upstream CYP450 monooxygenase (AuAP450) generated four new sesterterpene alcohols. Additionally, aspergilol A showed cytotoxic activities against MCF-7, MDA-MB231, and HepG2 cancer cells (IC 21.20-48.76 μM), and aspergilol B exhibited a cytotoxic effect on MCF-7 cells (IC 27.41 μM).
基因组挖掘 094102 使得发现一种多产物双功能萜烯合酶（BTS），AuAS。AuAS 的异源表达导致发现了五种新的倍半萜，而上游 CYP450 单加氧酶（AuAP450）的共表达则生成了四种新的倍半萜醇。此外，aspergilol A 对 MCF-7、MDA-MB231 和 HepG2 癌细胞具有细胞毒性活性（IC 21.20-48.76 μM），aspergilol B 对 MCF-7 细胞具有细胞毒性作用（IC 27.41 μM）。
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