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来源于 Streptomyces mobaraensis 的 Sestermobaraenes 的生物合成基因簇——香叶基二磷酸合酶和多功能倍半萜合酶的发现

The Biosynthetic Gene Cluster for Sestermobaraenes-Discovery of a Geranylfarnesyl Diphosphate Synthase and a Multiproduct Sesterterpene Synthase from Streptomyces mobaraensis.

机构信息

Kekulé-Institute of Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagk-Strasse 1, 53121, Bonn, Germany.

出版信息

Angew Chem Int Ed Engl. 2020 Nov 2;59(45):19961-19965. doi: 10.1002/anie.202010084. Epub 2020 Aug 31.

DOI:10.1002/anie.202010084
PMID:32749032
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7693059/
Abstract

A biosynthetic gene cluster from Streptomyces mobaraensis encoding the first cases of a bacterial geranylfarnesyl diphosphate synthase and a type I sesterterpene synthase was identified. The structures of seven sesterterpenes produced by these enzymes were elucidated, including their absolute configurations. The enzyme mechanism of the sesterterpene synthase was investigated by extensive isotope labeling experiments.

摘要

从 Streptomyces mobaraensis 中鉴定出一个生物合成基因簇，该基因簇编码了细菌香叶基香叶基二磷酸合酶和 I 型甾体合酶的首例。通过这些酶产生的七种甾体的结构得到了阐明，包括它们的绝对构型。通过广泛的同位素标记实验研究了甾体合酶的酶机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae5/7693059/9fe8e05ce6c8/ANIE-59-19961-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae5/7693059/3e5f8ea60d73/ANIE-59-19961-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae5/7693059/aa86b8452960/ANIE-59-19961-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae5/7693059/6334bd4c5b49/ANIE-59-19961-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae5/7693059/9fe8e05ce6c8/ANIE-59-19961-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae5/7693059/3e5f8ea60d73/ANIE-59-19961-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae5/7693059/aa86b8452960/ANIE-59-19961-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae5/7693059/6334bd4c5b49/ANIE-59-19961-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae5/7693059/9fe8e05ce6c8/ANIE-59-19961-g004.jpg

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