Department of Fish Pathology and Immunology, Stanisław Sakowicz Inland Fisheries Institute, Oczapowskiego 10, 10-719 Olsztyn, Poland.
Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland.
Pol J Vet Sci. 2020 Dec;23(4):611-618. doi: 10.24425/pjvs.2020.135808.
The aim of the experiment was to examine the effect of a diet enriched with Lactobacillus plantarum and/or β-glucan on the immune parameters in the juvenile tench (Tinca tinca). Fish were fed for 14 days different diets (phase 1 of the experiment), a dry commercial starter feed in the control group or the same feed supplemented with: 1% β-1,3/1,6-glucan in group G, 108 cfu L. plantarum g-1 in group L, 1% β-1,3/1,6-glucan + 108 cfu L. plantarum g-1 in group G+L. During consecutive 14 days all fish were fed the commercial feed alone (phase 2). The stimulating effects of the tested preparations was evaluated twice, at the end of each experimental phase. Dietary supplementation of β-1,3/1,6-glucan considerably improved the humoral innate immune response (activity of lysozyme and total Ig) and the pinocytotic activity of phagocytes. Supplement of L. plantarum improved the ability of the head kidney phagocytes (RBA) to carry out oxygen burst in L and G+L groups. A similar effect was observed for the killing activity of phagocytes (PKA) from the head kidney after the stimulation of A. hydrophila, and the effect persisted for two weeks after the commercial feed regime was resumed. A significant increase in the proliferative activity of B lymphocytes originating from the head kidney was observed in groups L and G+L. The study has revealed that the addition of the tested G+L synbiotic to dry diet stimulates the innate immune response mechanisms in the juvenile tench.
实验目的是研究富含植物乳杆菌和/或β-葡聚糖的饮食对幼年丁鱥（Tinca tinca）免疫参数的影响。鱼在 14 天内分别食用不同的饮食（实验的第一阶段）：对照组为干商业起始饲料，或相同的饲料中添加：G 组为 1%β-1,3/1,6-葡聚糖、L 组为 108cfuL.plantarumg-1、G+L 组为 1%β-1,3/1,6-葡聚糖+108cfuL.plantarumg-1。在连续 14 天内，所有鱼都单独食用商业饲料（第二阶段）。在实验的每一个阶段结束时，对测试制剂的刺激作用进行了两次评估。β-1,3/1,6-葡聚糖的饮食补充大大提高了体液固有免疫反应（溶菌酶和总 Ig 的活性）和吞噬细胞的胞饮作用。植物乳杆菌的补充提高了头肾吞噬细胞（RBA）在 L 和 G+L 组中进行氧爆发的能力。头肾吞噬细胞的杀伤活性（PKA）在刺激嗜水气单胞菌后也观察到了类似的效果，并且在恢复商业饲料方案后两周内仍然存在。头肾来源的 B 淋巴细胞的增殖活性在 L 和 G+L 组中显著增加。研究表明，将测试的 G+L 共生元添加到干饮食中可刺激幼年丁鱥的固有免疫反应机制。
