取代的乙烯基-BODIPY 作为硫醇选择性荧光探针，用于检测内质网中未折叠的蛋白质。
Substituted -vinyl-BODIPY as thiol-selective fluorogenic probes for sensing unfolded proteins in the endoplasmic reticulum.
机构信息
Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
出版信息
Chem Commun (Camb). 2021 Feb 19;57(14):1818-1821. doi: 10.1039/d0cc08160d.
A new type of thiol probes based on the meso-vinyl-BODIPY (VB) scaffold were developed. The monochloro-substituted VB1Cl exhibited the largest fluorescence enhancement (>200-fold) as well as high selectivity upon biological thiol sensing. VB1Cl was successfully applied for reporting the protein unfolding process under ER stress in living cells.
研究人员开发了一种基于中-乙烯基-BODIPY（VB）支架的新型硫醇探针。单氯取代 VB1Cl 在生物硫醇传感中表现出最大的荧光增强（>200 倍）和高选择性。VB1Cl 成功应用于报告活细胞内质网应激下蛋白质展开过程。
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