基于 BODIPY 的半胱氨酸和高半胱氨酸荧光开启探针。
BODIPY-based turn-on fluorescent probes for cysteine and homocysteine.
机构信息
Key Laboratory for Special Functional Materials of the Ministry of Education, Henan University, Kaifeng 475004, PR China.
Key Laboratory for Special Functional Materials of the Ministry of Education, Henan University, Kaifeng 475004, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Oct 5;203:77-84. doi: 10.1016/j.saa.2018.05.114. Epub 2018 May 29.
Cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) are interconnected and play essential roles in many biological processes. It is significant importance to detect these thiols for investigating their functions in cells and disease diagnosis. In this work, we have designed and synthesized two novel BODIPY-based turn-on fluorescent probes (BDP-Ph and BDP-R-Ph) carrying 4-methoxythiophenol moiety at meso position as good leaving group for highly selective detection of Cys and Hcy. Furthermore, the probes have been successfully applied to detect intracellular Cys and Hcy by fluorescent imaging in living cells.
半胱氨酸 (Cys)、同型半胱氨酸 (Hcy) 和谷胱甘肽 (GSH) 相互关联，在许多生物过程中发挥着重要作用。检测这些硫醇对于研究它们在细胞中的功能和疾病诊断具有重要意义。在这项工作中，我们设计并合成了两个新型基于 BODIPY 的荧光探针 (BDP-Ph 和 BDP-R-Ph)，它们在中位位置带有 4-甲氧基噻吩基团作为良好的离去基团，可高度选择性地检测 Cys 和 Hcy。此外，这些探针已成功应用于通过荧光成像在活细胞中检测细胞内的 Cys 和 Hcy。
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