Department of Chemistry, University of Ottawa, 10 Marie-Curie, Ottawa, ON, K1N 6N5, Canada.
Angew Chem Int Ed Engl. 2018 Sep 17;57(38):12390-12394. doi: 10.1002/anie.201805482. Epub 2018 Aug 24.
We report the development of YC23, a novel green BODIPY-based dimaleimide derivative that undergoes a fluorogenic addition reaction (FlARe) with a genetically encodable peptide tag (dC10α) that can be fused to a protein of interest (POI). We also demonstrate the application of this reaction for the fluorogenic labelling of a specific POI in bacterial lysate and in living mammalian cells.
我们报告了 YC23 的开发，这是一种新型的基于 BODIPY 的二马来酰亚胺衍生物，与可融合到感兴趣的蛋白质（POI）的遗传编码肽标签（dC10α）发生荧光加成反应（FlARe）。我们还展示了该反应在细菌裂解物和活哺乳动物细胞中对特定 POI 的荧光标记的应用。
