Departament of Dental Materials and Prosthesis, School of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café, s/n, Ribeirão Prêto, SP, 14040-904, Brazil.
Laboratory of Solid State Chemistry, Institute of Chemistry, University of Campinas (Unicamp), Cidade Universitária Barão Geraldo, Campinas, SP, 13083-970, Brazil.
Odontology. 2021 Jul;109(3):605-614. doi: 10.1007/s10266-020-00582-0. Epub 2021 Jan 22.
This study aimed to evaluate two methods of the incorporation of nanostructured silver vanadate decorated with silver nanoparticles (AgVO) into acrylic resin and characterize the profile of early and late microbial communities in class and family taxonomic level by pyrosequencing. The specimens were made by adding different concentrations of AgVO (1, 2.5, and 5%) to the heat-activated acrylic resin by two methods: vacuum spatulation (VS) and polymeric film (PF). A control group (0%) without AgVO was also obtained for both methods. After 24 h and 7 days of incubation in human saliva, biofilm samples were collected, DNA was extracted, and 16S rRNA genes were sequenced by the 454-Roche sequencing platform. Seventeen classes and 51 families of bacteria were identified. The abundance of Bacteroidia, Bacilli, Negativicutes, Fusobacteria and Betaproteobacteria classes decreased after 7 days of incubation, and Clostridia, Gammaproteobacteria, and unclassified bacteria increased. The Negativicutes and Betaproteobacteria classes were more abundant when the PF method was used, and Gammaproteobacteria was more abundant when VS was used. The incorporation of 5% AgVO promoted a reduction in the prevalence of Bacilli, Clostridia, Negativicutes, Betaproteobacteria, and unclassified bacteria, and increased Gammaproteobacteria. The addition of AgVO to acrylic resin altered the early and mature microbiome formed on the specimen surface, and the PF method presented a more favorable microbial profile than the VS method.
本研究旨在评估两种将纳米结构的银钒酸负载纳米银（AgVO）掺入丙烯酸树脂的方法，并通过焦磷酸测序法在纲和科分类水平上对早期和晚期微生物群落的特征进行分析。通过两种方法（真空搅拌[VS]和聚合薄膜[PF]）将不同浓度的 AgVO（1%、2.5%和 5%）添加到热激活丙烯酸树脂中制备标本。对于这两种方法，还获得了不含 AgVO 的对照组（0%）。在人类唾液中孵育 24 小时和 7 天后，收集生物膜样本，提取 DNA，并通过 454-Roche 测序平台对 16S rRNA 基因进行测序。共鉴定出 17 个纲和 51 个科的细菌。在孵育 7 天后，厚壁菌门、芽孢杆菌门、拟杆菌门、梭杆菌门和β变形菌门的丰度降低，而梭菌门、γ变形菌门和未分类细菌增加。当使用 PF 方法时，Negativicutes 和 Betaproteobacteria 类更丰富，而当使用 VS 方法时，Gammaproteobacteria 更丰富。掺入 5%AgVO 可降低 Bacilli、Clostridia、Negativicutes、Betaproteobacteria 和未分类细菌的流行率，并增加γ变形菌门的丰度。AgVO 对丙烯酸树脂的添加改变了标本表面形成的早期和成熟微生物组，PF 方法比 VS 方法呈现出更有利的微生物特征。
