Institute of Environmental Engineering, National Chiao-Tung University, Hsinchu 30010, Taiwan, ROC.
Department of Public Health, Chung-Shan Medical University, Taichung 402, Taiwan, ROC.
Int J Biol Macromol. 2021 Mar 15;173:211-218. doi: 10.1016/j.ijbiomac.2021.01.126. Epub 2021 Jan 20.
Fluoroquinolone antibiotics are widely used in human and veterinary medicine. However, untreated fluoroquinolone seriously threatens the ecosystem and human health. In this study, deep eutectic solvents (DESs) were applied for the hydrolysis of waste feathers, and the keratin particles (KPs) in a low-cost teabag were utilized to adsorb fluoroquinolone norfloxacin. Results showed that choline chloride/ethylene glycol DES rapidly hydrolyzed feathers within 10 min, and the undissolved particles effectively adsorbed norfloxacin. Adding KOH markedly shortened the hydrolysis time (6 min) and increased the adsorption ability of KPs. The optimum hydrolysis conditions were DES ratio of 1 g: 4.67 g, KOH of 35.68 g L, and temperature of 90 °C. When KP of 2 g L, norfloxacin of 25 mg L, and pH 7 were used, 94% of norfloxacin was removed in 60 min. A low-cost teabag effectively separated the KPs from the solution after adsorption and did not decrease the adsorption ability of the KPs. The Langmuir isotherm model well described the adsorption behavior of KPs (q = 79.36 mg g, R = 0.9972). In addition, acetone efficiently regenerated the exhausted KPs. The KPs maintained >80% of its adsorption ability after seven cycles of regeneration.
氟喹诺酮类抗生素广泛应用于人类和兽医医学。然而，未经处理的氟喹诺酮类抗生素严重威胁着生态系统和人类健康。在这项研究中，深共熔溶剂（DESs）被应用于羽毛的水解，并且利用一种低成本茶包中的角蛋白颗粒（KPs）来吸附氟喹诺酮类药物诺氟沙星。结果表明，氯化胆碱/乙二醇 DES 在 10 分钟内迅速水解羽毛，而未溶解的颗粒有效地吸附了诺氟沙星。添加 KOH 显著缩短了水解时间（6 分钟）并提高了 KPs 的吸附能力。最佳水解条件为 DES 比为 1 g:4.67 g，KOH 为 35.68 g·L-1，温度为 90°C。当使用 2 g·L-1 的 KP、25 mg·L-1 的诺氟沙星和 pH 值为 7 时，60 分钟内去除了 94%的诺氟沙星。低成本茶包在吸附后有效地将 KPs 从溶液中分离出来，并且不会降低 KPs 的吸附能力。Langmuir 等温吸附模型很好地描述了 KPs 的吸附行为（q=79.36 mg·g-1，R=0.9972）。此外，丙酮可以有效地再生耗尽的 KPs。经过七次再生循环后，KPs 的吸附能力保持在 80%以上。
