BioSystems & Integrative Sciences Institute (BioISI), Plant Functional Biology Centre, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253, Bragança, Portugal.
Microbiol Res. 2021 Apr;245:126693. doi: 10.1016/j.micres.2020.126693. Epub 2021 Jan 5.
A wide array of fungal endophytes is known to inhabit plant tissues and were recently recognized as essential for plant health. A better description of the scarcely known endophyte microbiota in olive tree phyllosphere is the first step for elucidating the microbial interactions that lead to olive disease establishment. In this work, the fungal endophytic community of the phyllosphere of different olive tree cultivars (Cobrançosa, Galega vulgar, Madural, Picual, Verdeal Transmontana) is revealed by using a metabarcoding strategy targeting ITS1 barcode. A total of 460 OTUs were obtained, increasing the broad view of fungal endophytes inhabiting the olive tree phyllosphere, in particular yeast endophytes. New endophytes were persistently found in all cultivar tissues. Different olive tree cultivars depicted distinct endophyte communities. Olive cultivars exhibited dissimilar amounts of fungi with distinct ecological functions, which could explain at least in part their differential susceptibility/tolerance to olive diseases.
已知广泛的真菌内生菌栖息在植物组织中，最近被认为对植物健康至关重要。更好地描述橄榄树叶际中鲜为人知的内生菌微生物群，是阐明导致橄榄树疾病发生的微生物相互作用的第一步。在这项工作中，通过使用靶向 ITS1 条码的代谢组学策略，揭示了不同橄榄树品种（Cobrançosa、Galega vulgar、Madural、Picual、Verdeal Transmontana）叶际的真菌内生群落。共获得 460 个 OTUs，增加了栖息在橄榄树叶际中的真菌内生菌的广泛视野，特别是酵母内生菌。新的内生菌在所有品种的组织中都持续存在。不同的橄榄树品种表现出不同的内生菌群落。橄榄品种表现出不同数量的具有不同生态功能的真菌，这至少可以部分解释它们对橄榄病的不同敏感性/耐受性。
